Development of an Enzyme Immunoassay for Urinary Pregnanediol-3-Glucuronide in a Female Giant Panda (Ailuropoda melanoleuca)

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概要

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• In order to enable monitoring of the reproductive status of the female giant panda after observation of estrus behavior, we developed an enzyme immunoassay (EIA) system for urinary pregnanediol-3-glucuronide (PdG), a progesterone metabolite, using commercial reagents and examined the changes in the urinary concentration of PdG in a female giant panda that showed pseudopregnancy and suspicious pseudopregnancy in 6 consecutive years. The developed EIA system had good reproducibility (intra- and interassay CVs 6.1% and 16.3%, respectively), good parallelism between the standard curve and the dose response curve of serial diluted samples and positive correlation (r=0.836) with the data for PdG in the same samples measured by gas chromatography. Urinary PdG in the female panda showed two phases of increase. The first elevation was observed immediately after estrus with the levels of PdG below 100 ng/Crmg, while the second phase was characterized by a drastic elevation above 100 ng/Crmg until the level began to decrease at the end of pseudopregnancy or suspicious pseudopregnancy. The length of the second phase had wider range than that of the first phase. In the present study, a new EIA assay system for urinary PdG in the female giant panda was developed, and we found that the length of the second phase is unstable in the pseudopregnant and suspicious pseudopregnant giant panda, in contrast with the unstable length of the first phase caused by delayed implantation in the pregnant giant panda.

著者

• Hama Natsuki

  Graduate School of Natural Science and Technology, Okayama University

• Kanemitsu Hideyasu

  Kobe Municipal Oji Zoo

• Tanikawa Michiyo

  Graduate School of Natural Science and Technology, Okayama University

• SHIBAYA Masami

  Graduate School of Natural Science and Technology, Okayama University

• SAKAMOTO Kensuke

  Kobe Municipal Oji Zoo

• OYAMA Yujiro

  Kobe Municipal Oji Zoo

• ACOSTA Tomas

  Graduate School of Natural Science and Technology, Okayama University

• ISHIKAWA Osamu

  Kobe Municipal Oji Zoo

• PENGYAN Wang

  China Research and Conservation Center for the Giant Panda

• OKUDA Kiyoshi

  Graduate School of Natural Science and Technology, Okayama University

• Pengyan Wang

  China Res. And Conservation Center For The Giant Panda Sichuan Chn

• Acosta Tomas

  Graduate School Of Natural Science And Technology Okayama University

• Okuda Kiyoshi

  Graduate School Of Natural Science And Technology Okayama University

• Okuda Kiyoshi

  Graduate School Of Natural Sci. And Technol. Okayama Univ.

• ACOSTA Tomas

  Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine

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