Changes in the Levels of Progesterone Receptor mRNA and Protein in the Bovine Corpus Luteum During the Estrous Cycle

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概要

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• Progesterone (P4) is synthesized in the luteal cells of many species. The objective of the present study was to determine the expression pattern of P4 receptor (PR) mRNA and the distribution of PR protein in the bovine corpus luteum (CL) during the estrous cycle. The gene expression of PR in the bovine CL throughout the estrous cycle was determined by real-time PCR analysis, and the PR protein expression was evaluated by immunohistochemistry. Messenger RNA of PR was clearly expressed in the CL throughout the estrous cycle. The level of PR mRNA in the CL was highest at the early stage of the estrous cycle and was higher at the mid and late stages than at the regressed stage (P<0.01). In regard to the distribution of PR, the protein was expressed in both small and large luteal cells and in vascular endothelial cells throughout the estrous cycle. These results suggest that P4 has a role in regulating luteal and endothelial cell function in the bovine CL, especially at the early luteal stage as an autocrine/paracrine regulator.

• 2010-04-01

著者

• SAKUMOTO Ryosuke

  Reproductive Biology Research Unit, National Institute of Agrobiological Sciences

• VERMEHREN Margarete

  Institute of Veterinary Anatomy, Histology and Embryology, Ludwig-Maximilians University

• KENNGOTT Rebecca

  Institute of Veterinary Anatomy, Histology and Embryology, Ludwig-Maximilians University

• OKUDA Kiyoshi

  Laboratory of Reproductive Endocrinology, Okayama University

• SINOWATZ Fred

  Institute of Veterinary Anatomy, Histology and Embryology, Ludwig-Maximilians University

• Okuda Kiyoshi

  Laboratory Of Reproductive Endocrinology Graduate School Of Natural Science And Technology Okayama U

• Sinowatz Fred

  Institute Of Veterinary Anatomy Histology And Embryology Ludwig-maximilians University

• Sakumoto Ryosuke

  Reproductive Biology Research Unit National Institute Of Agrobiological Sciences

• Kenngott Rebecca

  Institute Of Veterinary Anatomy Histology And Embryology Ludwig-maximilians University

• Vermehren Margarete

  Institute Of Veterinary Anatomy Histology And Embryology Ludwig-maximilians University

• Sakumoto Ryosuke

  Reproductive Biology Res. Unit National Inst. Of Agrobiological Sciences

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