ウシ形成期黄体において、プロスタグランジンF2αはプロジェステロン生成のためのリポプロテイン利用性を増加させる

概要

論文の詳細を見る
Luteal cells utilize lipoproteins (LIPs) for progesterone (P) synthesis. A luteolysin prostaglandin F2α (PGF 2α) has shown to prevent the LIPs utilization for steroidogenesis in bovine midcycle luteal cells. However, a luteolytic injection of PGF2α before Day 5 (Day 0=estrus) can not induce the regression of the corpus luteum (CL). This suggests that the intraluteal mechanism of PGF2 α action on the LIPs utilization in early CL is different from that of the mid luteal stage. Thus, this study aimed to investigate the interaction between PGF2 α and LIPs on the secretion of P and oxytocin (OT) from bovine early CL. An in vitro microdialysis system (MDS) was used as a model. Each bovine early CL (Days 3-4) was implanted with the MDS, and maintained in an organ culture chamber. The infusion of LIPs (1 μg/ml) stimulated P release during infusion, but had no effect on OT release. The infusion of PGF2α (10-5 M) stimulated both P and OT release during infusion. When LIPs were infused after PGF2α exposure, P and OT release continued to be stimulated until 3 h thereafter. The results show that PGF2α is capable of enhancing the LIPs utilization in early CL, thus support the concept that luteal PGF2α acts as a luteotropic promoter in bovine early CL.
日本繁殖生物学会の論文