様々なフタレート類におけるin vitroとin vivoの評価モデル間でみられるエストロゲン様活性の矛盾はCalbindin-D9kの発現と関連した現象として理解できる
スポンサーリンク
概要
- 論文の詳細を見る
Phthalates are suspected to disrupt the endocrine system, especially through estrogenic effects. In the present study, we investigated the effects of various phthalates and compared them with those of estrogenic compounds that disrupt the female reproductive system. To assess the effects of these phthalates, alteration of the Calbindin-D9k (CaBP-9k) gene was measured as a biomarker because rat CaBP-9k gene carries an estrogen response element (ERE) which is involved in estrogen responsiveness of the gene during the estrous cycle. In this study, phthalates were tested for estrogenic properties in in vitro and in vivo models. First, the E-Screen assay was used to measure the proliferation of MCF-7 cells, a human breast cancer cell line. Treatments with 17β-estradiol (E2; 9-fold) and 17α-estradiol (EE; 9-fold) induced MCF-7 cell proliferation at concentrations of 10-9 M. Phthalates induced an increase in MCF-7 proliferation at concentration of 10-6 M up to 10-4 M. Nbutyl benzyl phthalate (BBP; 6-fold vs. vehicle), dicyclohexyl phthalate (DCHP; 8-fold), 2-ethylhexyl phthalate (DEHP; 6-fold) and di-n-butyl phthalate (DBP; 7-fold) at the concentration of 10-4 M induced in an increase in MCF-7 proliferation after 6 d of treatment compared to vehicle. However, significant increase in MCF-7 proliferation was induced by diethyl phthalate (DEP). Second, we investigated the expression of CaBP-9k in the uterus of immature rats after oral treatment with BBP, DCHP, DEHP, DBP or DBP (600 mg/kg per day) in this in vivo model, because the immature rat model is highly sensitive to exposure to estrogenic chemicals. None of the phthalates induced the expression of CaBP-9k mRNA and its protein in the neonatal uterus as analysed by Northern and Western blot analyses, respectively. Although phthalates induced an increase in MCF-7 cell proliferation by an estrogenic effect, they could not induce CaBP-9k expression in the in vivo system, suggesting that the assays of estrogenic effects of various phthalates conducted in vitro and in vivo expression of CaBP-9k may produce conflicting results.
- 日本繁殖生物学会の論文
著者
-
MANABE Noboru
Research Unit of Animal Life Sciences, Animal Resource Science Centre, The University of Tokyo
-
JEUNG Eui-Bae
Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbu
-
Choi Kyung-chul
Department Of Obstetrics And Gynecology British Columbia Women's Hospital Child And Family Rese
-
HONG Eui-Ju
Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbu
-
Ji Youn-Kyu
Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbu
-
Hong Eui-ju
Laboratory Of Veterinary Biochemistry And Molecular Biology College Of Veterinary Medicine Chungbuk
関連論文
- Metabolic Conversion of Zearalenone to α-Zearalenol by Goat Tissues
- Estrogen Receptors are Involved in Xenoestrogen Induction of Growth Hormone in the Rat Pituitary Gland
- 異種移植したブタ卵巣組織における卵胞と卵母細胞の発育と成熟およびその体外成熟培養
- Differential Effects of Flutamide and Di-(2-ethylhexyl) phthalate on Male Reproductive Organs in a Rat Model
- 経胎盤ビスフェノールA(BPA)暴露が器官形成期のマウス胎仔におけるarylhydrocarbon受容体(AhR)とレチノイン酸受容体mRNA発現におよぼす影響
- 経胎盤的ビスフェノールA暴露がマウス胎仔におけるアリルヒドロカーボン受容体とその関連因子および生体異物代謝酵素の発現におよぼす影響
- ブタ卵胞における選択的閉鎖の制御機構:顆粒層細胞アポトーシスの制御
- ブタ卵胞閉鎖にともなうコネキシン43の発現と局在の推移
- ブタcellular FLICE-like inhibitory protein遺伝子のクローニングと発現解析
- Expression and Localization of cFLIP, an Anti-apoptotic Factor, in the Bovine Corpus Luteum
- Calbindin-D9k mRNA Expression in the Rat Uterus Following Exposure to Methoxychlor: A Comparison of Oral and Subcutaneous Exposure
- ICGNマウスにおける組織トランスグルタミナーゼ(tTG)の局在
- Expression and Function of Apoptosis Initiator FOXO3 in Granulosa Cells During Follicular Atresia in Pig Ovaries
- Surgical Experience of Gas-Containing Disk Herniation
- Dietary Calcium and 1,25-Dihydroxyvitamin D_3 Regulate Transcription of Calcium Transporter Genes in Calbindin-D9k Knockout Mice
- 様々なフタレート類におけるin vitroとin vivoの評価モデル間でみられるエストロゲン様活性の矛盾はCalbindin-D9kの発現と関連した現象として理解できる
- cFLIP Regulates Death Receptor mediated Apoptosis in an Ovarian Granulosa Cell Line by Inhibiting Procaspase-8 Cleavage
- Changes in Expression of Interleukin-6 Receptors in Granulosa Cells During Follicular Atresia in Pig Ovaries
- The Regulation of Ovarian Granulosa Cell Death by Pro- and Anti-apoptotic Molecules
- Expression and Localization of Fas Ligand and Fas During Atresia in Porcine Ovarian Follicles
- The Role of Interleukin-6 in the Regulation of Granulosa Cell Apoptosis during Follicular Atresia in Pig Ovaries
- Molecular Characteristics of Porcine Fas-associated Death Domain (FADD) and Procaspase-8
- ハンガリー在来ブタ(Mangalica)とLandrace雌ブタの排卵期における卵胞・卵母細胞の発育・成熟と生殖ホルモン分泌の比較
- Antibacterial and Antifungal Effects of Essential Oils from Coniferous Trees(Pharmacognosy)
- 未成熟マウス子宮においてカルシウム結合タンパク(CaBP-9k)の転写と翻訳は性ステロイドホルモンとその受容体によって特異的に制御される
- Influence of the prodrugs 5-fluorocytosine and CPT-11 on ovarian cancer cells using genetically engineered stem cells : tumor-tropic potential and inhibition of ovarian cancer cell growth
- Cell Growth of Ovarian Cancer Cells is Stimulated by Xenoestrogens through an Estrogen-Dependent Pathway, but Their Stimulation of Cell Growth Appears not to be Involved in the Activation of the Mitogen-Activated Protein Kinases ERK-1 and p38
- Antifungal Effect of Eugenol and Nerolidol against Microsporum gypseum in a Guinea Pig Model(Medicinal Chemistry)
- Calbindin-D_ mRNA Expression in the Rat Uterus Following Exposure to Methoxychlor : A Comparison of Oral and Subcutaneous Exposure
- The Biomarker and Endocrine Disruptors in Mammals
- 妊娠後期の母体に投与したBisphenol Aは母体および出産後の仔ラットの子宮におけるCalbindin-D9kのmRNAとタンパクの発現を増加させる
- Potential Estrogenic and Antiandrogenic Effects of Permethrin in Rats
- Maternal Exposure to Bisphenol A during Late Pregnancy Resulted in an Increase of Calbindin-D_ mRNA and Protein in Maternal and Postnatal Rat Uteri
- Conflict of Estrogenic Activity by Various Phthalates between In Vitro and In Vivo Models Related to the Expression of Calbindin-D_
- Differential Transcriptional and Translational Regulations of Calbindin-D_ by Steroid Hormones and Their Receptors in the Uterus of Immature Mice
- Molecular Characteristics of Porcine Fas-associated Death Domain (FADD) and Procaspase-8
- Growth and Maturation of Follicles and Oocytes Following Xenotransplantation of Porcine Ovarian Tissues and In Vitro Maturation
- Changes in Expression and Localization of Connexin 43 mRNA and Protein in Porcine Ovary Granulosa Cells during Follicular Atresia
- Effects of Exposure In Utero to Bisphenol A on the Expression of Aryl Hydrocarbon Receptor, Related Factors, and Xenobiotic Metabolizing Enzymes in Murine Embryos
- Effects of In Utero Exposure to Bisphenol A on mRNA Expression of Arylhydrocarbon and Retinoid Receptors in Murine Embryos
- Porcine (Sus scrofa) Cellular FLICE-like Inhibitory Protein (cFLIP) : Molecular Cloning and Comparison with the Human and Murine cFLIP
- Regulation Mechanism of Selective Atresia in Porcine Follicles : Regulation of Granulosa Cell Apoptosis during Atresia
- Comparison of Follicular and Oocyte Development and Reproductive Hormone Secretion during the Ovulatory Period in Hungarian Native Breed, Mangalica, and Landrace Gilts
- Effects of essential oil from Chamaecypris obtusa on the development of atopic dermatitis-like skin lesions and the suppression of Th cytokines
- Expression and Function of Apoptosis Initiator FOXO3 in Granulosa Cells During Follicular Atresia in Pig Ovaries
- Incorporation of β-amyloid protein through the bovine ileal epithelium before and after weaning : Model for orally transmitted amyloidoses