妊娠後期の母体に投与したBisphenol Aは母体および出産後の仔ラットの子宮におけるCalbindin-D9kのmRNAとタンパクの発現を増加させる
スポンサーリンク
概要
- 論文の詳細を見る
It has been reported that Calbindin-D9k (CaBP-9k) is rapidly and strongly induced by environmental estrogenic compounds, possibly through estrogen receptors (ERα) in the uterus of mammals. CaBP-9k can be evaluated as an early gene marker for assaying estrogenic effects of putative environmental chemicals in the rat uterus. This study was undertaken to investigate CaBP-9k mRNA and protein expression in the postnatal rat uterus following maternal exposure to 17β-estradiol (E2) and bisphenol A (BPA) during the neonatal period. Treatment with a high dose of BPA (600 mg/kg body weight (BW) per day) resulted in a 3-fold increase in CaBP-9k mRNA expression for 3 days, while a single dose of E2 (40 μg/kg BW per day) induced 2-fold increase of this gene in the maternal uterus. In an agreement with maternal CaBP-9k mRNA, postnatal CaBP-9k mRNA in the uterus increased 4-fold when treated with BPA (600 mg/kg BW per day). In addition, treatment with increasing concentrations of BPA resulted in significant increases in CaBP-9k protein in the maternal rat uterus. It is of interest that increasing doses of BPA induced a significant ERα mRNA increase in the postnatal uterus. Furthermore, immunohistochemistry revealed that treatment with BPA induced CaBP-9k protein in the maternal uterus. We demonstrated that maternal exposure to BPA during late pregnancy induced CaBP-9k mRNA and protein in maternal and postnatal rat uteri. These results suggest that rapid absorption and distribution of environmental estrogenic compounds occurs in maternal and neonatal rat uteri and these chemicals can easily pass though the placenta during pregnancy to affect postnatal reproductive functions.
- 日本繁殖生物学会の論文
著者
-
JEUNG Eui-Bae
Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbu
-
Choi Kyung-chul
韓国
-
Choi Kyung-chul
Department Of Obstetrics And Gynecology British Columbia Women's Hospital Child And Family Rese
-
Jeung Eui-bae
Laboratory Of Veterinary Biochemistry And Immunology College Of Veterinary Medicine Chungbuk Nationa
-
HONG Eui-Ju
Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbu
-
Choi Kyung-chul
Department Of Neurosurgery Wooridul Spine Hospital
-
CHO Kyung-Chul
Department of Obstetrics and Gynecology, British Columbia Childrens and Womens Hospital, British Col
-
Hong Eui-ju
Laboratory Of Veterinary Biochemistry And Molecular Biology College Of Veterinary Medicine Chungbuk
-
JEUNG Eui-Bae
Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National Universit
-
CHOI Kyung-Chul
Department of Obstetrics and Gynecology, British Columbia Children's and Women's Hospital, British Columbia Research Institute for Children's and Women's Health, University of British Columbia
関連論文
- Estrogen Receptors are Involved in Xenoestrogen Induction of Growth Hormone in the Rat Pituitary Gland
- Differential Effects of Flutamide and Di-(2-ethylhexyl) phthalate on Male Reproductive Organs in a Rat Model
- Duodenal and Renal Transient Receptor Potential Vanilloid 6 Is Regulated by Sex-Steroid Hormones, Estrogen and Progesterone, in Immature Rats
- Calbindin-D9k mRNA Expression in the Rat Uterus Following Exposure to Methoxychlor: A Comparison of Oral and Subcutaneous Exposure
- Surgical Experience of Gas-Containing Disk Herniation
- Dietary Calcium and 1,25-Dihydroxyvitamin D_3 Regulate Transcription of Calcium Transporter Genes in Calbindin-D9k Knockout Mice
- 様々なフタレート類におけるin vitroとin vivoの評価モデル間でみられるエストロゲン様活性の矛盾はCalbindin-D9kの発現と関連した現象として理解できる
- Antibacterial and Antifungal Effects of Essential Oils from Coniferous Trees(Pharmacognosy)
- 未成熟マウス子宮においてカルシウム結合タンパク(CaBP-9k)の転写と翻訳は性ステロイドホルモンとその受容体によって特異的に制御される
- Influence of the prodrugs 5-fluorocytosine and CPT-11 on ovarian cancer cells using genetically engineered stem cells : tumor-tropic potential and inhibition of ovarian cancer cell growth
- Cell Growth of Ovarian Cancer Cells is Stimulated by Xenoestrogens through an Estrogen-Dependent Pathway, but Their Stimulation of Cell Growth Appears not to be Involved in the Activation of the Mitogen-Activated Protein Kinases ERK-1 and p38
- Antifungal Effect of Eugenol and Nerolidol against Microsporum gypseum in a Guinea Pig Model(Medicinal Chemistry)
- Calbindin-D_ mRNA Expression in the Rat Uterus Following Exposure to Methoxychlor : A Comparison of Oral and Subcutaneous Exposure
- The Biomarker and Endocrine Disruptors in Mammals
- 妊娠後期の母体に投与したBisphenol Aは母体および出産後の仔ラットの子宮におけるCalbindin-D9kのmRNAとタンパクの発現を増加させる
- Potential Estrogenic and Antiandrogenic Effects of Permethrin in Rats
- Maternal Exposure to Bisphenol A during Late Pregnancy Resulted in an Increase of Calbindin-D_ mRNA and Protein in Maternal and Postnatal Rat Uteri
- Conflict of Estrogenic Activity by Various Phthalates between In Vitro and In Vivo Models Related to the Expression of Calbindin-D_
- Differential Transcriptional and Translational Regulations of Calbindin-D_ by Steroid Hormones and Their Receptors in the Uterus of Immature Mice
- Degradation of Bisphenol A by White Rot Fungi, Stereum hirsutum and Heterobasidium insulare, and Reduction of Its Estrogenic Activity(Analytical Biochemistry)
- ラットを用いた合成ピレスロイド系殺虫剤ペルメスリンのエストロゲン様および抗アンドロゲン様活性の評価
- Effects of essential oil from Chamaecypris obtusa on the development of atopic dermatitis-like skin lesions and the suppression of Th cytokines
- Tissue-Specific Expression of the Calcium Transporter Genes TRPV5, TRPV6, NCX1, and PMCA1b in the Duodenum, Kidney and Heart of Equus caballus
- Dietary Calcium and 1,25-Dihydroxyvitamin D3 Regulate Transcription of Calcium Transporter Genes in Calbindin-D9k Knockout Mice