Tissue-Specific Expression of the Calcium Transporter Genes TRPV5, TRPV6, NCX1, and PMCA1b in the Duodenum, Kidney and Heart of Equus caballus
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概要
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Calcium transporter genes, such as transient receptor potential cation channel subfamily V members 5/6 (TRPV5/6), Na+/Ca2+ exchanger 1 (NCX1), and plasma membrane calcium-transporting ATPase 1b (PMCA1b), are essential for maintaining homeostasis and metabolizing Ca2+ ions. The TRPV5 and TRPV6 proteins play an important role in Ca2+ absorption, and NCX1 and PMCA1b are both critical for intracellular calcium homeostasis. In this study, the tissue-specific mRNA and protein expression of these calcium transporter genes in the duodenum, kidney and heart of the horse (Equus caballus) was examined using reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis. The tissue localization of these calcium transporters was also investigated using immunohistochemistry. The results showed that TRPV5 mRNA was highly expressed in the kidney but was scarce in the duodenum and heart. TRPV6 mRNA levels were similar in all the tissues. NCX1 and PMCA1b were both highly expressed in the heart, but no difference in NCX1 and PMCA1b mRNA expressions was observed in the duodenum and kidney. The aspect of protein expression was similar with mRNA expression data. Localization of calcium transporter genes were detected in cytoplasm of enterocytes in duodenum, the cytosol of the distal convoluted tubules in the kidney, and within the cardiac muscle cells of the heart. Based on these results, calcium transport genes appear to be expressed in horse tissues at levels similar to those observed in other vertebrates.
著者
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Choi Kyung-chul
Laboratory Of Veterinary Biochemistry And Immunology College Of Veterinary Medicine Chungbuk Nationa
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Jung Eui-man
Laboratory Of Veterinary Biochemistry And Molecular Biology College Of Veterinary Medicine Chungbuk
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Jeung Eui-bae
カナダ
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Jeung E‐b
Chungbuk National Univ. Chungbuk Kor
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Jeung Eui-bae
Laboratory Of Veterinary Biochemistry And Immunology College Of Veterinary Medicine Chungbuk Nationa
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YANG Hyun
Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbu
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HWANG Inho
Laboratory of Veterinary Biochemistdry and Molecular Biology, College of Veterinary Medicine, Chungb
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JUNG Eui-Man
Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University
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CHOI Kyung-Chul
Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University
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HWANG Inho
Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University
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