Conditioning of Karyoplasts for Producing Somatic Nuclear Transferred Gonadal Germ Cells in Domestic Chickens
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概要
- 論文の詳細を見る
The objective of this study was to establish a protocol for generating karyoplasts that can be used to produce somatic nuclear transferred gonadal germ cells (snt-GGCs) in domestic chickens. Karyoplasts were produced by centrifuging cultured fibroblasts from 10-day-old chick embryos at 10000×g in the presence of 1.0 μg/ml cytochalasin B. The number of karyoplasts was significantly (P<0.05) higher and the diameters of the karyoplasts were significantly (P<0.05) smaller when fibroblasts were centrifuged for 60 min than for 10 or 30 min. It was possible to generate snt-GGCs by electrofusion of GGCs with karyoplasts produced from cryopreserved or serum-starved fibroblasts. These results indicate that karyoplasts generated from 10-day-old chick embryos can be used to produce snt-GGCs even after cryopreservation and serum starvation of the fibroblasts.
- 日本繁殖生物学会の論文
- 2008-06-01
著者
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KANAI Yukio
Graduate School of Life and Environmental Sciences, University of Tsukuba
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TAJIMA Atsushi
Graduate School of Life and Environmental Sciences, University of Tsukuba
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Atsushi Tajima
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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KOHARA Yusaku
Graduate School of Life and Environmental Sciences, University of Tsukuba
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MINEMATSU Takeo
Graduate School of Life and Environmental Sciences, University of Tsukuba
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AIKAWA Takeshi
Graduate School of Life and Environmental Sciences, University of Tsukuba
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KANAI Yukio
Tsukuba University
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Kohara Yusaku
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Aikawa Takeshi
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Kanai Y
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Kanai Yukio
Univ. Of Tsukuba
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Kanai Yukio
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Minematsu Takeo
Department Of Gerontological/wound Care Nursing The University Of Tokyo
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Minematsu Takeo
Transgenic Animal Research Center National Institute Of Agrobiological Sciences
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Tajima Atsushi
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Tajima Atsushi
Graduate School Of Life And Environmental Science University Of Tsukuba
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