Germinal Vesicle Stages in Pig Follicular Oocytes Collected by Different Methods
スポンサーリンク
概要
- 論文の詳細を見る
In Exp. 1 oocytes were collected from follicles (4-6 mm) in ovaries from prepubertal gilts by aspiration of the follicles with a syringe or dissection from healthy follicles. After aspiration some oocytes with compact cumulus and granulosa cells were selected. In Exp.2 oocytes were collected from follicles (4 mm, 5-6 mm and 7-8 mm) of gilts on the 18th-19th day of the cycle. Germinal vesicle (GV) stages of oocytes were examined and categorized into GV1, GV2, GV3 and GV4. In Exp. 1 when collected by aspiration, a significantly lower percentage of oocytes (45%) was arrested at the GV1 stage than oocytes from dissected follicles (78%). However, after morphological selection of oocytes, the percentage (58%) significantly increased. In Exp 2, significantly higher percentages (86-90%) of oocytes from follicles of 5-8 mm in diameter were at the GV1 stage than oocytes from follicles in diameter of 4 mm (24%) and atretic follicles (57%). These results indicate that by aspiration some oocytes are collected from follicles that were inadequate to maintain arrest at GV1, but significantly higher percentages of oocytes at GV1 can be obtained after morphological selection of oocytes or isolation from large follicles of gilts on the 18th-19th day of the cycle.
- 日本繁殖生物学会の論文
- 1997-11-01
著者
-
Nagai Takashi
Department of Internal Medicine, Public Tomioka General Hospital
-
Nagai Takashi
Department Of Animal Breeding And Reproduction National Institute Of Livestock And Grassland Science
-
EBIHARA Misa
Laboratory for Bio-resource Science, Research and Development Center, Nippon Meat Packers INC., Tsuk
-
ONISHI Akira
Department of Animal Genetics and Breeding, National Institute of Animal Industry, Tsukuba, Ibaraki
-
KUBO Masanori
Pathological Diagnosis Laboratory, Systemic Diagnosis Research Division, National Institute of Anima
-
Onishi Akira
Department Of Animal Genetics And Breeding National Institute Of Animal Industry
-
Kubo Masanori
Pathological Diagnosis Laboratory Systemic Diagnosis Research Division National Institute Of Animal
-
Nagai Takashi
Department Of Animal Production Tohoku National Agricultural Experiment Station
-
Ebihara Misa
Laboratory For Bio-resource Science Research And Development Center Nippon Meat Packers Inc.
-
ONISHI Akira
Department of Animal Breeding and Genetics, National Institute of Animal Industry
関連論文
- Introduction of Various Vietnamese Indigenous Pig Breeds and Their Conservation by Using Assisted Reproductive Techniques
- Reactive Hypoglycemia Associated with Transient Hyperthyroidism in the Background of the Prediabetic Stage
- 沖縄島漫湖饒波川のマングローブ域における大型底生生物の分布と出現量
- Introduction of Various Vietnamese Indigenous Pig Breeds and Their Conservation by Using Assisted Reproductive Techniques
- Effect of Cryoprotectant Composition on In Vitro Viability of In Vitro Fertilized and Cloned Bovine Embryos Following Vitrification and In-Straw Dilution
- 4Cで冷蔵保存された精巣上体から採取・凍結・融解した精子を用いたブタの繁殖の試み
- Deterioration of Retinopathy After Starting Interferon Therapy for Chronic Hepatitis C with Diabetic Triopathy
- Secondary Hemochromatosis Accompanied by Diabetes Mellitus and Hypogonadism.
- Seroconversion of Glutamic Acid Decarboxylase Antibodies in a Patient Initially Diagnosed as Having Type 2 Diabetes Mellitus
- Metformin Use in an Obese Diabetic Patient from Weeks 1 to 21 of Pregnancy.
- Sarcoid Granulomatous Interstitial Nephritis Accompanied by Type 2 Diabetes Mellitus
- Graves' Disease Accompanied by Anti-myeloperoxidase Antibody-related Nephropathy and Autoimmune Hepatitis
- 17α-Hydroxylase Deficiency Accompanied by Adrenal Myelolipoma
- Developmental Competence of Cat Oocytes from Ovaries Stored at Various Temperature for 24 h
- シクロヘキシミドへの曝露が電気的に活性化したネコ卵母細胞の発育に及ぼす効果
- Taxonomy of the Terrestrial Crab Geothelphusa tenuimanus (Crustacea: Decapoda: Brachyura: Potamidae) of Okinawa Island, Central Ryukyus, Japan
- 培養卵丘細胞をドナー核とした体外成熟ブタ卵母細胞の核移植後の発生能力
- Evaluation of Developmental Competence of In Vitro-produced Porcine Embryos Based on the Timing, Pattern and Evenness of the First Cleavage and Onset of the Second Cleavage
- Intracytoplasmic Sperm Injection in the Pig : Where is the Problem?
- Nuclear and Cytoplasmic Maturation In Vitro of Porcine Oocytes
- Effect of Bezafibrate or Pravastain on Serum Lipid Levels and Albuminuria in MIDDM Patients
- Effect of Nephropathy on the Composition of Apolipoprotein-Containing Particles in NIDDM
- Prader-Willi Syndrome with Elevated Follicle Stimulating Hormone Levels and Diabetes Mellitus
- Diabetes Mellitus Accompanied by Nonocclusive Colonic Ischemia
- Candida Glabrata Fungemia After Amputation of an Infected Diabetic Foot
- Cervical Spinal Epidural Abscess and Perirenal Abscess Associated with Diabetes Mellitus
- An Overwhelming Postsplenectomy Infection Syndrome Associated with Diabetes M ellitus
- M ultiple Organ Failure Followed by Intrauterine Klebsiella Pneumoniae Infection Associated with Diabetes M ellitus
- An approach for conservation of pig resources by xenograft of gonadal tissue to mice
- Effect of Centrifugation Treatment before Vitrification on the Viability of Porcine Mature Oocytes and Zygotes Produced In Vitro
- 初期胞状卵胞から採取した牛卵母細胞の生存と体外成長に及ぼす酸素濃度の影響
- A Comparison of Cryotop and Solid Surface Vitrification Methods for the Cryopreservation of In Vitro Matured Bovine Oocytes
- Germinal Vesicle Stages in Pig Follicular Oocytes Collected by Different Methods
- Interspecies Nuclear Transfer Embryos Reconstructed from Cat Somatic Cells and Bovine Ooplasm
- Health Status and Productive Performance of Somatic Cell Cloned Cattle and Their Offspring Produced in Japan
- CONTROL OF JET IMPINGEMENT HEAT TRANSFER BY A WAKE FLOW BEHIND AN ARRAY OF CIRCULAR CYLINDERS
- The Birth of Piglets from Embryos Cultured for 3 to 5 Days in a Simple Salt Solution Lacking Glucose
- Larval Development of a Pontoniine Shrimp, Periclimenes brevicarpalis (Crustacea : Decapoda : Palaemonidae) Reared in the Laboratory
- 無血清成熟培地へのシステアミンの添加がウシ未成熟卵子の体外成熟率,体外受精率およびその後の発生率に及ぼす影響
- Pregnancy complicated with Alport syndrome : A good obstetric outcome and failure to diagnose an infant born to a mother with Alport syndrome by umbilical cord immunofluorescence staining
- Developmental Competence of Cat Oocytes from Ovaries Stored at Various Temperature for 24h
- Effect of Cycloheximide on In Vitro Development of Electrically Activated Feline Oocytes
- Development of Porcine Embryos Reconstructed by Nuclear Transfer of Cultured Cumulus Cells into In Vitro Matured and Enucleated Oocytes
- Ejaculated Boar Spermatozoa Can Penetrate Oocytes Maturing In Vitro without Preincubation
- Solids Circulation Rate in Screen-Bottomed Spouted Bed with Draft-Tube
- The Birth of Piglets from Embryos Cultured for 3 to 5 Days in a Simple Salt Solution Lacking Glucose
- Nitrogen Uptake by Paddy Rice (Oryza sativa L.) from ^N Labelled Coated Urea and Ammonium Sulfate
- HUMAN NEUROPHYSINS DURING PREGNANCY, PUERPERIUM AND MENSTRUAL CYCLES
- Growth, Prey Consumption and Food Assimilation Efficiency in a Mantid, Paratenodera angustipennis (S.)
- Germinal Vesicle Stages in Pig Follicular Oocytes Collected by Different Methods
- Evaluation of Developmental Competence of In Vitro-produced Porcine Embryos Based on the Timing, Pattern and Evenness of the First Cleavage and Onset of the Second Cleavage
- 4Cで冷蔵保存された精巣上体から採取・凍結・融解した精子を用いたブタの繁殖の試み
- Effect of β-Merchaptoethanol on the Preimplantation Development of Bovine Embryos Fertilized In Vitro
- 91. Concerning the Clinical Significance of Air Myelography
- In Situ Lymph Dynamic Characterization through Lymph Nodes in Rabbit Hind Leg : Special Reference to Nodal Inflammation
- Subclinical Cushing's Disease Accompanied by Malignant Hypertension and Diabetes Mellitus
- 66. Clincal Investigation on Ossification of Postero-Longitudinal Ligamentum around the Cervical Vertebrae with Cord Compression. (Proceedings of the XXVth Annual Meeting of the Japan Neurosurgical Society)
- Constant transmission of mitochondrial DNA in intergeneric cloned embryos reconstructed from swamp buffalo fibroblasts and bovine ooplasm
- Real-time imaging of the lymphatic channels and sentinel lymph nodes of the stomach using contrast-enhanced ultrasonography with Sonazoid in a porcine model
- SPOUTED BED WITH A DRAFT-TUBE WITHOUT GAS INLET NOZZLE OR ORIFICE
- Downregulation of Histone Methyltransferase Genes SUV39H1 and SUV39H2 Increases Telomere Length in Embryonic Stem-like Cells and Embryonic Fibroblasts in Pigs
- A case of an autonomously functioning thyroid nodule associated with low triiodothyronine concentrations.
- A simple technique for production of chimeric mouse embryos.
- A Case of Adrenocortical Adenoma Producing 11-Deoxycorticosterone (DOC) Independent of ACTH Control.
- A case of pheochromocytoma accompanied with augmentation of growth hormone due to administration of growth hormone releasing hormone.
- The reactions of (trichlorostannato)rhodates(III) with trichlorostannate(II) in hydrochloric acid solutions.
- A case of T-cell non-hodgkin lymphoma with hypercalcemia due to massive bone metastatic lesions.
- Downregulation of Histone Methyltransferase Genes SUV39H1 and SUV39H2 Increases Telomere Length in Embryonic Stem-like Cells and Embryonic Fibroblasts in Pigs
- Mycophenolate mofetil versus intravenous cyclophosphamide for induction treatment of proliferative lupus nephritis in a Japanese population : a retrospective study