Efficient Transfection of Chicken Blastoderms in vivo by Lipofection and Electroporation Using Green Fluorescent Protein Gene as a Marker

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概要

• 論文の詳細を見る
• 2000-07-25

著者

• TAGAMI Takahiro

  National Institute of Livestock and Grassland Science

• Sano Akiko

  Transgenic Animal Research Center National Institute Of Agrobiological Sciences

• NAITO Mitsuru

  National Institute of Agrobiological Sciences

• MATSUBARA Yuko

  National Institute of Agrobiological Sciences

• HARUMI Takashi

  National Institute of Agrobiological Sciences

• Harumi Takashi

  Animal Genome Research Unit National Institute Of Agrobiological Sciences

• Matsubara Yuko

  Transgenic Animal Research Center National Institute Of Agrobiological Sciences

• SANO Akiko

  National Institute of Agrobiological Sciences

• Naito M

  Transgenic Animal Research Center National Institute Of Agrobiological Sciences

• Naito Mitsuru

  Animal Development And Differentiation Research Unit National Institute Of Agrobiological Sciences

• Tagami Takahiro

  National Agricultural And Food Research Organization (naro) Institute Of Livestock And Grassland Science (nilgs)

関連論文

• Depletion of Primordial Germ Cells (PGCs) by X-irradiation to Extraembryonic Region of Chicken Embryos and Expression of Xenotransplanted Quail PGCs
• Effects of Busulfan Sustained-release Emulsion on Depletion and Repopulation of Primordial Germ Cells in Early Chicken Embryos
• Restriction of Germline Proliferation by Soft X-ray Irradiation of Chicken Embryos and its Application to Chimera Production
• Polymerase chain reaction detection of single nucleotide polymorphisms in the chicken mitochondrial D-loop region
• Effect of the strain combination of the donor and recipient on the production efficiency of W-bearing sperm in mixed-sex germline chimeric chickens
• New Screening Test for Male Germline Chimeric Chickens by Polymerase Chain Reaction Using Shingle Nucleotide Polymorphism Detection Primers
• Molecular Cloning and Sequence Analysis of the Gene Encoding the Collagenase from Cytophaga sp. L43-1 Strain
• Purification and Properties of Collagenase from Cytophaga sp. L43-1 Strain
• In vitro culture of testicular and ovarian gonocytes obtained from 19-day incubated chicken embryos and subsequent colonization into gonads of recipient embryos
• Interspecies Nuclear Transfer Embryos Reconstructed from Cat Somatic Cells and Bovine Ooplasm
• Cryopreservation of Primordial Germ Cells (PGCs) from White Leghorn Embryos Using Commercial Cryoprotectants
• Mitochondrial DNA analysis of Nepalese domestic dwarf cattle Lulu
• Substantial Evidence to Localize the Developmental Origin of Primordial Germ Cells in the Chicken
• Substantial Evidence to Localize the Developmental Origin of Primordial Germ Cells in the Chicken
• Preferential Migration of Transferred Primordial Germ Cells to Left Germinal Ridge of Recipient Embryos in Chickens
• Intense Expression of GFP Gene in Gonads of Chicken Embryos by Transfecting Circulating Primordial Germ Cells in vitro and in vivo
• Genetic Factors Affect the Number of Circulating Primordial Germ Cells in Early Chick Embryos
• Morphological Characteristics of Symmetrically Developed Ovaries in a 17-day Incubated Chicken Embryo
• Long Term in vitro Culture of Chicken Primordial Germ Cells Isolated from Embryonic Blood and Incorporation into Germline of Recipient Embryo
• Effects of Soft X-Ray Irradiation to Stage X Blastoderm on Restriction of Proliferation of Primordial Germ Cells in Early Chicken Embryos
• Culture of Chicken Embryos Obtained from the Anterior Region of the Magnum of the Oviduct after Removing a Thin Layer of Dense Albumen Capsule from the Ovum
• An Attempt at Fusing Primordial Germ Cell with Embryonic Blood Cell in Chickens Using Inactivated Sendai Virus or Electrical Stimulation
• Effect of Electrode Size on Transfection Efficiency of Chicken Stage X Blastoderm by in vivo Electroporation
• A Simple Method for Sexing Chicken Embryos at Stage X
• Efficient Gene Transfer into Early Chicken Embryos by Electroporation of Stage X Blastoderms in Vivo, Applying Electric Pulses Vertically to the Blastoderm Layer
• Effect of Mytomycin C Treatment on Feeder Cells and the Proliferation of Cultured Chicken Primordial Germ Cells Isolated from Embryonic Blood
• Fate of the Donor Blastodermal Cells Derived from the Central Desc, Marginal Zone and Area Opaca Transferred into the Recipient Embryos
• Efficient Transfection of Chicken Blastoderms in vivo by Lipofection and Electroporation Using Green Fluorescent Protein Gene as a Marker
• Efficient Transfection of Chicken Blastoderms in vivo by Lipofection and Electroporation Using Green Fluorescent Protein Gene as a Marker
• Foreign Gene Expression in the Gonads of Chimaeric Chicken Embryos by Transfer of Primordial Germ Cells Transfected in vitro by Lipofection for 24 Hours
• Foreign Gene Expression in the Gonads of Chimaeric Chicken Embryos by Transfer of Primordial Germ Cells Transfected in vitro by Lipofection for 24 Hours
• Development of avian embryo manipulation techniques and their application to germ cell manipulation
• The role of gravity in chick embryogenesis
• Differentiation of female chicken primordial germ cells into spermatozoa in male gonads
• Conservation of a Threatened Indigenous Fowl (Kureko Dori) Using the Germline Chimeras Transplanted from Primordial Germ Cells
• In vitro Culture of Testicular and Ovarian Gonocytes Obtained from 19-day Incubated Chicken Embryos and Subsequent Colonization into Gonads of Recipient Embryos
• Viability and Functionality of Primordial Germ Cells after Freeze-thaw in Chickens
• A Simple Culture Method of Chicken Blastodermal Cells for Germline Transmission
• A Method for Cryopreserving Semen from Yakido Roosters Using N-Methylacetamide as a Cryoprotective Agent
• Foreign Gene Expression in the Gonads of Chimaeric Chicken Embryos by Transfer of Primordial Germ Cells Transfected in vitro by Lipofection for 24 Hours
• Expression of GFP Gene in Cultured PGCs Isolated from Embryonic Blood and Incorporation into Gonads of Recipient Embryos
• Allele-specific PCR typing and sequencing of the mitochondrial D-loop region in four layer breeds
• Constant transmission of mitochondrial DNA in intergeneric cloned embryos reconstructed from swamp buffalo fibroblasts and bovine ooplasm
• Expression of GFP Gene in Cultured PGCs Isolated from Embryonic Blood and Incorporation into Gonads of Recipient Embryos
• Detection of the EGFP sequence in breast muscle of 3-year-old chicken after transfection using sonoporation
• Substantial Evidence to Localize the Developmental Origin of Primordial Germ Cells in the Chicken
• Comparative Proteomic Analysis of Liver Mitochondrial Proteins Derived from Cloned Adult Pigs Reconstructed with Meishan Pig Fibroblast Cells and European Pig Enucleated Oocytes

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