Comparative Proteomic Analysis of Liver Mitochondrial Proteins Derived from Cloned Adult Pigs Reconstructed with Meishan Pig Fibroblast Cells and European Pig Enucleated Oocytes
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概要
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Somatic cell nuclear transfer (SCNT) has been exploited in efforts to clone and propagate valuable animal lineages. However, in many instances, recipient oocytes are obtained from sources independent of donor cell populations. As such, influences of potential nuclear-cytoplasmic incompatibility, post SCNT, are largely unknown. In the present study, alterations in mitochondrial protein levels were investigated in adult SCNT pigs produced by microinjection of Meishan pig fetus fibroblast cells into enucleated matured oocytes (maternal Landrace genetic background). Mitochondrial fractions were prepared from liver samples by mechanical homogenization and differential centrifugation. Liver mitochondria were then subjected to two-dimensional difference gel electrophoresis (2-D DIGE). Protein expression changes were confirmed with a volume ratio greater than 2 fold (P<0.05). 2-D DIGE analysis further revealed differential expression of three proteins between the Meishan (n=3) and Landrace (n=3) breeds. Differential expression patterns of 16 proteins were detected in SCNT pig liver tissue (n=3) when compared with Meishan control samples. However, none of the 16 proteins correlated with the three differentially expressed Meishan and Landrace liver mitochondrial proteins. In summary, alteration of mitochondrial protein expression levels was observed in adult SCNT pigs that did not reflect the breed difference of the recipient oocytes. Comparative proteomic analysis represents an important tool for further studies on SCNT animals.
- 2012-04-01
著者
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TAGAMI Takahiro
National Institute of Livestock and Grassland Science
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ONISHI Akira
National Institute of Agrobiological Sciences
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TAKEDA Kumiko
National Institute of Livestock and Grassland Science
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Nakamura Yoshiaki
Res. Fellow Of The Japan Soc. For The Promotion Of Sci. Jpn
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Chikuni Koichi
National Food Research Institute Ministry Of Agriculture Forestry And Fisheries
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Tasai Mariko
Department Of Animal Breeding And Reproductive National Institute Of Livestock And Grassland Science
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TASAI Mariko
National Institute of Livestock and Grassland Science, National Agricultural Research Organization
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Tagami T
Division Of Earth And Planetary Sciences Graduate School Of Science Kyoto University
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Hanada Hirofumi
National Institute Of Livestock And Grassland Science National Agricultural Research Organization
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Tatsumi Kouichi
Fac. Of Agriculture Shinshu Univ. Jpn
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Takeda Kumiko
Fac. Of Agriculture Shinshu Univ. Jpn
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IWAMOTO Masaki
Prime Tech Ltd.
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OE Mika
National Institute of Livestock and Grassland Science
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Pinkert Carl
Department Of Comparaive Medicine University Of Alabama At Birmingham
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Nirasawa Keijiro
National Agricultural And Food Res. Organization (naro) Inst. Of Livestock And Grassland Sci. (nilgs) Ibaraki ...
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Pinkert Carl
Department Of Pathobiology College Of Veterinary Medicine Auburn University
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Nakamura Yoshiaki
National Agricultural And Food Research Organization (naro) Institute Of Livestock And Grassland Science (nilgs)
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HANADA Hirofumi
Laboratory of Animal Breeding, Tokyo University of Agriculture
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Tasai Mariko
National Agricultural And Food Research Organization (naro) Institute Of Livestock And Grassland Science (nilgs)
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Hanada Hirofumi
Laboratory Of Animal Breeding Tokyo University Of Agriculture
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Oe Mika
National Agricultural And Food Res. Organization (naro) Inst. Of Livestock And Grassland Sci. (nilgs) Ibaraki ...
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Oe Mika
National Agricultural And Food Research Organization (naro) Institute Of Livestock And Grassland Science (nilgs)
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Tagami Takahiro
National Agricultural And Food Research Organization (naro) Institute Of Livestock And Grassland Science (nilgs)
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Onishi Akira
National Inst. Of Agrobiological Sciences (nias) Ibaraki 305-8602 Jpn
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Onishi Akira
National Institute Of Agrobiological Sciences (nias)
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TAKEDA Kumiko
National Agricultural and Food Research Organization (NARO), Institute of Livestock and Grassland Science (NILGS)
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CHIKUNI Koichi
National Agricultural and Food Research Organization (NARO), Institute of Livestock and Grassland Science (NILGS)
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