X-irradiation Removes Endogenous Primordial Germ Cells (PGCs) and Increases Germline Transmission of Donor PGCs in Chimeric Chickens
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概要
- 論文の詳細を見る
Primordial germ cells (PGCs) are embryonic precursors of germline cells with potential applications in genetic conservation, transgenic animal production and germline stem cell research. These lines of research would benefit from improved germline transmission of transplanted PGCs in chimeric chickens. We therefore evaluated the effects of pretransplant X-irradiation of recipient embryos on the efficacy of germline transmission of donor PGCs in chimeric chickens. Intact chicken eggs were exposed to X-ray doses of 3, 6 and 9 Gy (dose rate = 0.12 Gy/min) after 52 h of incubation. There was no significant difference in hatching rate between the 3-Gy-irradiated group and the nonirradiated control group (40.0 vs. 69.6%), but the hatching rate in the 6-Gy-irradiated group (28.6%) was significantly lower than in the control group (P<0.05). No embryos irradiated with 9 Gy of X-rays survived to hatching. X-irradiation significantly reduced the number of endogenous PGCs in the embryonic gonads at stage 27 in a dose-dependent manner compared with nonirradiated controls. The numbers of endogenous PGCs in the 3-, 6- and 9-Gy-irradiated groups were 21.0, 9.6 and 4.6% of the nonirradiated control numbers, respectively. Sets of 100 donor PGCs were subsequently transferred intravascularly into embryos irradiated with 3 Gy X-rays and nonirradiated control embryos. Genetic cross-test analysis revealed that the germline transmission rate in the 3-Gy-irradiated group was significantly higher than in the control group (27.5 vs. 5.6%; P<0.05). In conclusion, X-irradiation reduced the number of endogenous PGCs and increased the germline transmission of transferred PGCs in chimeric chickens.
- 日本繁殖生物学会の論文
著者
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Usui Fumitake
Faculty Of Agriculture Shinshu University
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Kagami Hiroshi
Faculty Of Agriculture Shinshu University
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Ono Tamao
Faculty Of Agriculture Shinshu University
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Nakamura Yoshiaki
Res. Fellow Of The Japan Soc. For The Promotion Of Sci. Jpn
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Tagami Takahiro
Animal Breeding And Reproduction Research Team National Institute Of Livestock And Grassland Science
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MORI Takafumi
Faculty of Agriculture, Shinshu University
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Miyahara Daichi
Faculty Of Agriculture Shinshu University
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NAKAMURA Yoshiaki
Division of Germ Cell Biology, National Institute for Basic Biology, National Institute of Natural Sciences, Aichi 444-8787, Japan
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TAKEDA Kumiko
Animal Breeding and Reproduction Research Division, National Institute of Livestock and Grassland Science, Ibaraki 305-0901, Japan
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NIRASAWA Keijiro
Animal Breeding and Reproduction Research Division, National Institute of Livestock and Grassland Science, Ibaraki 305-0901, Japan
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