Emulsifying and Oil-binding Properties of Bovine Serum Albumin and Its Enzymatic Hydrolyzate
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概要
- 論文の詳細を見る
Bovine serum albumin (BSA) was hydrolyzed with proteases, and the emulsifying and oil-binding properties of the hydrolyzates were then studied. By limiting hydrolysis with trypsin, the emulsifying activity index (EAI) increased about 40% above its original level. In order to find out which peptides contributed to the good emulsifying properties of the hydrolyzate, the peptides adsorbed onto the emulsified oil globule surface were extracted and analyzed. SDS-polyacrylamide gel electrophoresis showed that a peptide with a molecular weight of about 24 kDa was preferentially adsorbed onto the oil surface. This 24 kDa peptide was found to be fairly hydrophobic and corresponds to the third domain of BSA, residues 377-582. In spite of the preferential adsorption characteristics of the 24 kDa peptide, this peptide itself had a lower EAI value than that of the whole hydrolyzate. The contribution of some small hydrophilic peptides, as well as the 24 kDa peptide, to the good emulsifying properties of the BSA hydrolyzate was suggested.
- 社団法人日本農芸化学会の論文
- 1993-06-23
著者
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Saito M
Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology
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Saito Masayoshi
National Food Research Institute Ministry Of Agriculture Forestry And Fisheries Tsukuba
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Chikuni K
National Food Res. Inst. Ministry Of Agriculture Forestry And Fisheries Ibaraki Jpn
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Chikuni Koichi
National Food Research Institute Ministry Of Agriculture Forestry And Fisheries
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SASAKI Mami
RIKEN Plant Science Center
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Shimizu Makoto
School of Food and Nutritional Sciences, The University of Shizuoka
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MONMA Michiko
National Food Research Institute
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Monma M
National Food Research Institute Maff
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Saito Masayoshi
National Food Research Institute Ministry Of Agriculture Forestry And Fisheries
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Shimizu Makoto
School Of Food And Nutritional Sciences University Of Shizuoka
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CHIKUNI Koichi
National Agricultural and Food Research Organization (NARO), Institute of Livestock and Grassland Science (NILGS)
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SAITO Masayoshi
National Food Research Institute
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