The Difference of Subcellular Localization between β-Conglycinin and Glycinin in Developing Soybean Cotyledons(Biological Chemistry)

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概要

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• Subcellular localization of β-conglycinin and glycinin in developing soybean cotyledons was examined by sucrose density gradient ultracentrifugation (SDG-UC). Two major bands appeared at densities of 1.05 and 1.15 in the SDG-UC. They were the microbody fraction and rER fraction, respectively, from the distribution of marker enzymes, electron microscopic observation, and the effects of MgCl_2 on the profile of SDG-UC. In the soluble and microbody fractions, α and α' subunits of β-conglycinin were major polypeptides. The amounts of β-conglycinin and glycinin associated with rER were roughly equal. Β-Conglycinin was found in the soluble and microbody fractions as well as the rER fraction while glycinin was mostly localized in the rER fraction.

• 社団法人日本農芸化学会の論文
• 1988-03-23

著者

• Sugimoto Toshio

  National Food Research Institute

• SAIO Kyoko

  National Food Research Institute

• 本 敏男

  神戸大学自然科学研究科

• MONMA Michiko

  National Food Research Institute

• Sugimoto T

  Hayashibara Biochemical Laboratories Inc.

• Monma M

  National Food Research Institute Maff

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