A Method for the Separation and Detection of Proteases Using Substrate-containing Gel Isoelectric Focusing Electrophoresis
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概要
- 論文の詳細を見る
We developed an accurate method to separate and detect proteases using isoelectric focusing electrophoresis. The simultaneous addition of 0.3% octyl-D-glucopylanoside or 8M urea to acrylamide gel containing gelatin and of cytochrome c and pl marker proteins to the sample solution prevented interaction between protease and substrate during electrophoresis. Picogram to nanogram quantities of commercial proteases, papain, chymotrypsin, and proteinase K, were detected at the estimative isoelectric point of these proteases.
- 社団法人日本農芸化学会の論文
- 1995-04-23
著者
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Sato T
Akita Prefectural Univ. Akita
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Sugimoto T
Plant Nutrition Laboratory Faculty Of Agriculture Kobe University
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Sugimoto T
Univ. Tsukuba Tsukuba Jpn
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Oji Yoshikiyo
Plant Nutrition Laboratory Faculty Of Agriculture Kobe University
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Oji Yoshikiyo
Faculty Of Agriculture Kobe University
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Yamauchi Yasuo
Laboratory Of Plant Biotechnololgy Faculty Of Agriculture Tottori University
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Sugimoto Toshio
Faculty of Agriculture, Kobe University
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Yamauchi Yasuo
Science of Biological Resources, Graduate School of Science and Technology, Kobe University
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Sugimoto T
Hayashibara Biochemical Laboratories Inc.
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