Purification and Characterization of a Novel Enzyme, Maltooligosyl Trehalose Trehalohydrolase, from Arthrobacter sp. Q36
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概要
- 論文の詳細を見る
A novel enzyme, maltooligosyl trehalose trehalohydrolase from Arthrobacter sp. Q36 was purified from a cell-free extract to an electrophoretically pure state by successive column chromatography on Sepabeads FP-DA13, Butyl-Toyopearl 650M, DEAE-Toyopearl 650S, and Toyopearl HW-55S. The enzyme specifically catalyzed the hydrolysis of the α-1,4-glucosidic linkage that bound the maltooligosyl and trehalose moieties of maltooligosyl trehalose. The K_m of the enzyme for maltosyl trehalose, maltotriosyl trehalose, maltotetraosyl trehalose, and maltopentaosyl trehalose was 5.5 mM, 4.6 mM, 7.0 mM, and 4.2 mM, respectively. The enzyme had a molecular mass of 62,000 by SDS-polyacrylamide gel electrophoresis and a pI of 4.1 by gel isoelectrofocusing. The N-terminal amino acid of the enzyme was threonine. The enzyme showed the highest activity at pH 6.5 and 45℃, and was stable from pH 5.0 to 10.0 and up to 45℃. The activity was inhibited by Hg^<2+>, Cu^<2+>, Fe^<2+>, and Zn^<2+>.
- 社団法人日本農芸化学会の論文
- 1995-12-23
著者
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Chaen Hiroto
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Chaen H
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Chaen Hiroto
Hayashibara Biochemical Laboratories Inc.
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Kurimoto Masashi
Hayashibara Biochemical Laboratories Inc. Fujisaki Institute
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Kurimoto Masashi
Hayashibara Biochemical Laboratories
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SUGIMOTO Toshiyuki
Hayashibara Biochemical Laboratories
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TSUJISAKA Yoshio
Hayashibara Institute Corp.
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MITSUZUMI Hitoshi
Hayashibara Biochemical Laboratories, Inc.
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KUBOTA Michio
Hayashibara Biochemical Laboratories, Inc.
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NAKADA TETSUYA
Hayashibara Biochemical Laboratories Inc.
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Mitsuzumi Hitoshi
Hayashibara Biochemical Laboratories Inc.
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Sugimoto Toshiyuki
Hayashibara Biochemical Laboratories Inc.
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Nakada T
Amase Institute Hayashibara Biochemical Laboratories Inc.
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Sugimoto T
Plant Nutrition Laboratory Faculty Of Agriculture Kobe University
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Sugimoto T
Univ. Tsukuba Tsukuba Jpn
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Kubota Michio
Hayashibara Biochemical Laboratories Inc.
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Kurose Mayumi
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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MARUTA Kazuhiko
Hayashibara Biochemical Laboratories, Inc.
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Sonoda Tomohiko
Amase Institute Hayashibara Biochemical Laboratories Inc.
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Maruta K
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Chaen Hiroto
Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
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