Hyper Expression of Kojibiose Phosphorylase Gene and Trehalose Phosphorylase Gene from Thermoanaerobacter brockii ATCC35047 in Bacillus subtilis and Selaginose Synthesis Utilizing Two Phosphorylases(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
The kojibiose phosphorylase (KP) gene and trehalose phosphorylase (TP) gene from Thermo-anaerobacter brockii ATCC35047 were intracellularly hyper-expressed under the control of the Bacillus amyloliquefaciens α-amylase promoter in Bacillus subtilis. The production yields were estimated to be 2.1g of KP and 4.9g of TP per liter of medium. Selaginose, non-reducing trisaccharide, was synthesized from trehalose utilizing the recombinant KP and TP from B. subtilis. Selag-inose was not hydrolyzed by salivary amylase, artificial gastric juice, pancreatic amylase, or small intestinal enzymes.
- 社団法人日本生物工学会の論文
- 2005-09-25
著者
-
Chaen Hiroto
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
-
Chaen Hiroto
Glycoscience Institute Hayashibara Biochemical Laboratories Inc.
-
WATANABE Hikaru
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
-
FUKUDA Shigeharu
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
-
Chaen H
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
-
Fukuda S
Biomedical Inst. Res. Center Hayashibara Biochemical Laboratories Inc.
-
Fukuda Shigeharu
Glycoscience Institute Hayashibara Biochemical Laboratories Inc.
-
Kohguchi Michihiro
Fujisaki Institute Hayashibara Biochemical Laboratories Inc.
-
Kurimoto M
Amase Institute Hayashibara Biochemical Laboratories Inc.
-
Kibata Masayoshi
Hayashibara Biochemical Laboratories Inc.
-
Watanabe H
林原生物化学研究所
-
Watanabe Hikaru
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
-
Yamamoto Takuo
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
-
NISHIMOTO Tomoyuki
Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
-
Maruta Kazuhiko
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
-
Nishimoto Tomoyuki
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
-
Kubota Michio
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
-
Chaen Hiroto
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
-
Yamamoto Takuo
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
-
Mukai Kazuhisa
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
-
Yamashita Hiroshi
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
-
Chaen Hiroto
Glycoscience Inst. Res. Center Hayashibara Biochemical Laboratories Inc.
-
Kubota Michio
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
-
Kurose Mayumi
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
-
Mukai Kazuhisa
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
-
Kubota M
Hayashibara Biochemical Laboratories Inc.
-
Fukuda Shigeharu
Fujisaki Institute Hayashibara Biochemical Laboratories Inc.
-
Kubota M
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
-
Kurimoto M
Fujisaki Institute Hayashibam Biochemical Laboratories Inc.
-
Nishimoto Tomoyuki
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
-
Maruta K
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
-
Yamashita Hiroshi
Amase Institute Hayashibara Biochemical Laboratories Inc.
-
Fukuda Shigeharu
Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
-
Yamamoto Takuo
Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
-
Chaen Hiroto
Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
関連論文
- Improved yields of cyclic nigerosylnigerose from starch by pretreatment with a thermostable branching enzyme(BIOCHEMICAL ENGINEERING)
- Isolation and Identification of Antimicrobial Compounds in Brazilian Propolis
- Immuno-potentiating Effects of the Antler-shaped Fruiting Body of Ganoderma lucidum (Rokkaku-Reishi)
- Lumin, a Cyanine Dye, Enhances Interleukin 12-Dependent Interferon Gamma Production by Lipopolysaccharide-Stimulated Mouse Splenocytes
- Effect of Royal Jelly Diet on the Testicular Function of Hamsters
- Identification of a Collagen Production-promoting Factor from an Extract of Royal Jelly and Its Possible Mechanism
- Royal Jelly Inhibits the Production of Proinflammatory Cytokines by Activated Macrophages
- Promoting Effect of Kaempferol on the Differentiation and Mineralization of Murine Pre-osteoblastic Cell Line MC3T3-E1(Food & Nutrition Science)
- Tryptanthrin Inhibits Interferon-γ Production by Peyer's Patch Lymphocytes Derived from Mice That Had Been Orally Administered Staphylococcal Enterotoxin
- Preventive Effect of Coriandrum sativum (Chinese Parsley) on Aluminum Deposition in ICR Mice
- Trehalose Ingestion Modifies Mucosal Immune Responses of the Small Intestine in Mice
- Cell differentiation and apoptosis of monocytic and promyelocytic leukemia cells (U-937 and HL-60) by tryptanthrin, an active ingredient of Polygonum tinctorium Lour
- Antibacterial action of tryptanthrin and kaempferol, isolated from the indigo plant (Polygonum tinctorium Lour.), against Helicobacter pylori-infected Mongolian gerbils
- Renal carcinogenesis induced by ferric nitrilotriacetate in mice, and protection from it by Brazilian propolis and Artepillin C
- Isolation and Identification of Compounds from Brazilian Propolis which Enhance Macrophage Spreading and Mobility
- Metastasis-Promoting Activity of a Novel Molecule, Ag 243-5, Derived from Mycoplasma, and the Complete Nucleotide Sequence
- Localization of α-Glucosidases I, II, and III in Organs of European Honeybees, Apis mellifera L., and the Origin of α-Glucosidase in Honey
- Anti-inflammatory and Immunomodulatory Properties of 2-Amino-3H-phenoxazin-3-one(Pharmacognosy)
- Purification and Substrate Specificity of Honeybee, Apis mellifera L., α-Glucosidase III(Biochemistry & Molecular Biology)
- Synthesis of Glycosyl-trehaloses by Cyclomaltodextrin Glucanotransferase through the Transglycosylation Reaction
- Effect of Dietary Cyclic Nigerosylnigerose on Intestinal Immune Functions in Mice
- Enzymatic Synthesis of a Novel Trisaccharide, Glucosyl Lactoside
- Production of Isocyclomaltopentaose from Starch Using Isocyclomaltooligosaccharide Glucanotransferase
- Cloning, Sequencing, and Expression of the Genes Encoding an Isocyclomaltooligosaccharide Glucanotransferase and an α-Amylase from a Bacillus circulans Strain
- A Novel Glucanotransferase from a Bacillus circulans Strain That Produces a Cyclomaltopentaose Cyclized by an α-1,6-Linkage
- Bioavailability of Glucosyl Hesperidin in Rats
- Suppression of Apolipoprotein B Secretion from HepG2 Cells by Glucosyl Hesperidin
- Acceptor Recognition of Kojibiose Phosphorylase from Thermoanaerobacter brockii : Syntheses of Glycosyl Glycerol and myo-Inositol(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Acceptor Specificity of Trehalose Phosphorylase from Thermoanaerobacter brockii : Production of Novel Nonreducing Trisaccharide, 6-O-α-D-Galactopyranosyl Trehalose(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Glucosyl Hesperidin Lowers Serum Triglyceride Level in Hypertriglyceridemic Subjects through the Improvement of Very Low-Density Lipoprotein Metabolic Abnormality
- Hyper Expression of Kojibiose Phosphorylase Gene and Trehalose Phosphorylase Gene from Thermoanaerobacter brockii ATCC35047 in Bacillus subtilis and Selaginose Synthesis Utilizing Two Phosphorylases(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Effects of Glucosyl Hesperidin on Serum Lipids in Hyperlipidemic Subjects : Preferential Reduction in Elevated Serum Triglyceride Level
- Gene Encoding a Trehalose Phosphorylase from Thermoanaerobacter brockii ATCC 35047(Biochemistry & Molecular Biology)
- Enzymatic Synthesis of Kojioligosaccharides Using Kojibiose Phosphorylase
- Enzymatic Synthesis of Novel Oligosaccharides from L-Sorbose, Maltose, and Sucrose Using Kojibiose Phosphorylase
- Action of a Thermostable Trehalose Synthase from Thermus aquaticus on Sucrose
- Synthesis by an α-Glucosidase of Glycosyl-trehaloses with an Isomaltosyl Residue
- Purification and Characterization of a Thermostable Trehalose Synthase from Thermus aquaticus
- Purification and Properties of a Novel Enzyme, Trehalose Synthase, from Pimelobacter sp.R48
- Purification and Characterization of Thermostable Maltooligosyl Trehalose Trehalohydrolase from the Thermoacidophilic Archaebacterium Sulfolobus acidocaldarius
- Purification and Characterization of Cyclic Maltosyl-(1→6)-Maltose Hydrolase and α-Glucosidase from an Arthrobacter globiformis Strain
- Antimicrobial activity of Polygonum tinctorium Lour : extract against oral pathogenic bacteria
- 6-α-Glucosyltransferase and 3-α-Isomaltosyltransferase from Bacillus globisporus N75
- Cloning and Sequencing of Trehalose Biosynthesis Genes from Rhizobium sp. M-11
- Purification and Characterization of Thermostable Maltooligosyl Trehalose Synthase from the Thermoacidophilic Archaebacterium Sulfolobus acidocaldarius
- Purification and Characterization of a Novel Enzyme, Maltooligosyl Trehalose Trehalohydrolase, from Arthrobacter sp. Q36
- Purification and Properties of a Novel Enzyme, Maltooligosyl Trehalose Synthase, from Arthrobacter sp. Q36
- Formation of Trehalose from Maltooligosaccharides by a Novel Enzymatic System
- Purification and Characterization of Two Forms of Maltotetraose-forming Amylase from Pseudomonas stutzeri(Biological Chemistry)
- The Effect of AgK114 on Wound Healing(Highlighted paper selected by Editor-in-chief,Molecular and Cell Biology)
- Distribution of a Novel Protein AgK114 Expression in the Normal Tissues of Adult Mice : Dual Expression of AgK114 and Growth Hormone in Anterior Pituitary Cells(Endocrinology)
- Suppressive Effect of Trehalose on Acrylamide Formation from Asparagine and Reducing Saccharides
- Existence of a Novel Enzyme Converting Maltose into Trehalose
- Autocrine Interferon-β Stimulation Augments Nitric Oxide Production by Mouse Macrophage J774A.1 Cells Infected with Herpes Simplex Virus Type 1
- Enhancement of Thermostability of Kojibiose Phosphorylase from Thermoanaerobacter brockii ATCC35047 by Random Mutagenesis(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Interaction between Trehalose and Alkaline-Earth Metal Ions
- Cyclic Tetrasaccharide-Synthesizing Enzymes from Arthrobacter globiformis A19
- Enzymatic Synthesis of Glycosyl Cyclic Tetrasaccharide with 6-α-Glucosyltransferase and 3-α-Isomaltosyltransferase(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Cloning and Sequencing of Kojibiose Phosphorylase Gene from Thermoanaerobacter brockii ATCC35047(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Synthesis of 3-O-β-N-Acetylglucosaminyl Cyclic Tetrasaccharide through a Lysozyme-catalyzed Transfer Reaction(Biochemistry & Molecular Biology)
- Transglycosylation of Glycosyl Residues to Cyclic Tetrasaccharide by Bacillus stearothermophilus Cyclomaltodextrin Glucanotransferase Using Cyclomaltodextrin as the Glycosyl Donor(Biochemistry & Molecular Biology)
- Production of Cyclic Tetrasaccharide from Starch Using a Novel Enzyme System from Bacillus glob isporus C11
- Substrate Specificity and Subsite Affinities of Honeybee α-Glucosidase II
- Carbohydrate Structures of the Glycoprotein Allergen Cry j I from Japanese Cedar (Cryptomeria japonica) Pollen
- Enzymatic Properties of Recombinant Kojibiose Phosphorylase from Caldicellulosiruptor saccharolyticus ATCC43494
- Inhibitory Effects of 2-Amino-3H-phenoxazin-3-one on the Melanogenesis of Murine B16 Melanoma Cell Line
- Purification and Characterization of Glucosyltransferase and Glucanotransferase Involved in the Production of Cyclic Tetrasaccharide in Bacillus globisporus C11(Biochemistry & Molecular Biology)
- Cloning and Sequencing of the Genes Encoding Cyclic Tetrasaccharidesynthesizing Enzymes from Bacillus globisporus C11(Biochemistry & Molecular Biology)
- Cloning and Sequencing of the β-Fructofuranosidase gene from Bacillus sp.
- Trehalose-producing Operon treYZ from Arthrobacter ramosus S34
- Purification and Characterization of Cyclic Maltosyl-(1→6)-Maltose Hydrolase and α-Glucosidase from an Arthrobacter globiformis Strain
- Identification of the Novel Membrane-Associated Protein AgK114 on Hamster Keratinocytes Recognized by a Monoclonal Antibody K114(Biochemistry/Molecular Biology)
- Lactosucrose Inhibits Body Fat Accumulation in Rats by Decreasing Intestinal Lipid Absorption
- Effects of Dietary Lactosucrose (4^G-β-D-galactosylsucrose) on the IgE Response in Mice
- Prolonged Stability of Natural Human Interferon Alpha (IFN-α) Lozenges Using Anhydrous Maltose as an Excipient
- Cloning, Sequencing and Expression of the Genes Encoding Cyclic α-Maltosyl-(1→6)-maltose Hydrolase and α-Glucosidase from an Arthrobacter globiformis Strain
- Antiviral Effect of Polygonum tinctorium Lour. Extracts on Virus-Infected Cells
- Enzymatic Properties of Recombinant Kojibiose Phosphorylase from Caldicellulosiruptor saccharolyticus ATCC43494
- Preventive Effect of Coriandrum sativum (Chinese Parsley) on Aluminum Deposition in ICR Mice
- Isolation and Identification of Anti-Helicobacter pylori Compounds from Polygonum tinctorium Lour.
- Anti-Helicobacter pylori Compounds in Brazilian Propolis
- Purification and Characterization of Highly Branched α-Glucan–Producing Enzymes from Paenibacillus sp. PP710
- Purification and Characterization of Highly Branched α-Glucan-Producing Enzymes from Paenibacillus sp. PP710