Cyclic Tetrasaccharide-Synthesizing Enzymes from Arthrobacter globiformis A19
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概要
- 論文の詳細を見る
A bacterial strain Arthrobacter globiformis A19 producing cyclic tetrasaccharide (CTS) was isolated from soil. The enzymes, 6-α-glucosyltransferase (6GT) and 3-α-isomaltosyltransferase (IMT), involved in the synthesis of CTS were purified to homogeneity. The molecular and enzymatic properties of IMT from A. globiformis were similar to those of enzymes from Bacillus globisporus C11 and N75. Arthrobacter 6GT had a smaller molecular mass of 108 kDa and a higher optimum pH of 8.4 than the enzymes from strains of B. globisporus. The genes for IMT (ctsY) and 6GT (ctsZ) were cloned from the genome of A. globiformis A19. The two genes linked together in tandem and formed a gene cluster, ctsYZ. Both of the gene products showed similarities to α-glucosidases belonging to glycoside hydrolase family 31, and conserved two aspartic acids corresponding to the putative catalytic residues of the family enzymes. The enzymatic system for the production of CTS consisting of 6GT and IMT might be widespread among bacteria.
- 社団法人 日本農芸化学会の論文
- 2004-12-23
著者
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Kurimoto Masashi
Amase Institute, Hayashibara Biochemical Laboratories Inc.
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FUKUDA Shigeharu
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
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Fukuda S
Biomedical Inst. Res. Center Hayashibara Biochemical Laboratories Inc.
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Fukuda Shigeharu
Glycoscience Institute Hayashibara Biochemical Laboratories Inc.
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Kohguchi Michihiro
Fujisaki Institute Hayashibara Biochemical Laboratories Inc.
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Kurimoto M
Amase Institute Hayashibara Biochemical Laboratories Inc.
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Kibata Masayoshi
Hayashibara Biochemical Laboratories Inc.
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Maruta Kazuhiko
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
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Kubota Michio
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
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Tsujisaka Yoshio
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
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Mukai Kazuhisa
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
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Taniguchi Yoshifumi
Biomedical Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
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Kubota Michio
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Kurose Mayumi
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Mukai Kazuhisa
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Kubota M
Hayashibara Biochemical Laboratories Inc.
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Fukuda Shigeharu
Fujisaki Institute Hayashibara Biochemical Laboratories Inc.
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SATOUCHI Kazuhiro
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
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Kubota M
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Tsujisaka Y
Biomedical Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Kurimoto M
Fujisaki Institute Hayashibam Biochemical Laboratories Inc.
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Maruta K
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Satouchi Kazuhiro
Amase Institute Hayashibara Biochemical Laboratories Inc.
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Taniguchi Yoshifumi
Biomedical Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Fukuda Shigeharu
Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
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