Enhancement of Thermostability of Kojibiose Phosphorylase from Thermoanaerobacter brockii ATCC35047 by Random Mutagenesis(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
Random mutation by error-prone PCR was introduced into kojibiose phosphorylase from Thermoanaerobacter brockii ATCC35047. One thermostable mutant enzyme, D513N, was isolated. The D513N mutant enzyme showed an optimum temperature of 67.5-70℃ (the wild type, 65℃), and thermostability up to 67.5℃ (the wild type, up to 60℃). The half-lives of D513N were estimated to be 135 h at 60℃, 110min at 70℃ and 6min at 75℃, respectively. They were about 1.6-fold, 7-fold and 6-fold longer than those of the wild-type enzyme, respectively.
- 社団法人日本生物工学会の論文
- 2005-08-25
著者
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Kurimoto Masashi
Amase Institute, Hayashibara Biochemical Laboratories Inc.
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FUKUDA Shigeharu
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
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Fukuda S
Biomedical Inst. Res. Center Hayashibara Biochemical Laboratories Inc.
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Fukuda Shigeharu
Glycoscience Institute Hayashibara Biochemical Laboratories Inc.
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Kohguchi Michihiro
Fujisaki Institute Hayashibara Biochemical Laboratories Inc.
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Kurimoto M
Amase Institute Hayashibara Biochemical Laboratories Inc.
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Kibata Masayoshi
Hayashibara Biochemical Laboratories Inc.
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Yamamoto Takuo
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Kubota Michio
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
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Tsujisaka Yoshio
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
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Yamamoto Takuo
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
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Mukai Kazuhisa
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
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Yamashita Hiroshi
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
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Taniguchi Yoshifumi
Biomedical Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
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Kubota Michio
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Kurose Mayumi
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Mukai Kazuhisa
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Kubota M
Hayashibara Biochemical Laboratories Inc.
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Fukuda Shigeharu
Fujisaki Institute Hayashibara Biochemical Laboratories Inc.
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Kubota M
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Tsujisaka Y
Biomedical Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Kurimoto M
Fujisaki Institute Hayashibam Biochemical Laboratories Inc.
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Maruta K
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Taniguchi Yoshifumi
Biomedical Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Yamashita Hiroshi
Amase Institute Hayashibara Biochemical Laboratories Inc.
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Fukuda Shigeharu
Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
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Yamamoto Takuo
Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
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