Enzymatic Properties of Recombinant Kojibiose Phosphorylase from Caldicellulosiruptor saccharolyticus ATCC43494
スポンサーリンク
概要
- 論文の詳細を見る
One kojibiose phoshorylase (KP) homolog gene was cloned from Caldicellulosiruptor saccharolyticus ATCC43494. Recombinant KP from C. saccharolyticus (Cs-KP) expressed in Escherichia coli showed highest activity at pH 6.0 at 85 °C, and was stable from pH 3.5 to 10.0 and up to 85 °C for phosphorolysis. Cs-KP showed higher productivity of kojioligosaccharides of DP≧4 than KP from Thermoanaerobacter brockii ATCC35047.
著者
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Aga Hajime
Glycoscience Institute, Hayashibara Biochemical Laboratories, Inc.
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Chaen Hiroto
Glycoscience Institute, Hayashibara Biochemical Laboratories, Inc.
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Fukuda Shigeharu
Glycoscience Institute, Hayashibara Biochemical Laboratories, Inc.
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Fukuda Shigeharu
Glycoscience Institute Hayashibara Biochemical Laboratories Inc.
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Yamamoto Takuo
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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NISHIMOTO Tomoyuki
Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
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Chaen Hiroto
Glycoscience Inst. Res. Center Hayashibara Biochemical Laboratories Inc.
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NISHIO-KOSAKA Mio
Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
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IZAWA Seisuke
Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
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Nishimoto Tomoyuki
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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