Acceptor Recognition of Kojibiose Phosphorylase from Thermoanaerobacter brockii : Syntheses of Glycosyl Glycerol and myo-Inositol(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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概要
- 論文の詳細を見る
The glucosyl transfer reaction of kojibiose phosphorylase (KP; EC 2.4.1.230) was examined using glycerol or myo-inositol as an acceptor. In the case of glycerol, KP produced two main transfer products: saccharides A and B. The structure of saccharide A was O-α-D-glucopyranosyl-(1→1)-glycerol and that of saccharide B was O-α-D-glucopyranosyl-(1→2)-O-α-D-glucopyranosyl-(1→1)-glycerol. These results show that KP transferred a glucose residue to the hydroxyl group at position 1 of glycerol. On the other hand, when myo-inositol was used as an acceptor, KP produced four transfer products: saccharides 1-4. The structures of saccharides 1 and 2 were O-α-D-glucopyranosyl-(1→1)- and O-α-D-glucopyranosyl-(1→5)-myo-inositol, respectively; those of saccharides 3 and 4 were O-α-D-glucopyranosyl-(1→2)-O-α-D-glucopyranosyl-(1→1)- and O-α-D-glucopyranosyl-(1→2)-O-α-D-glucopyranosyl-(1→5)-myo-inositol, respectively. KP transferred a glucose residue to the hydroxyl group at position 1 or 5 of myo-inositol. On the basis of the structures of their glucosyl transfer products, glycerol and myo-inositol were found to have a common structure with three hydroxyl groups corresponding to the hydroxyl group of the glucose molecule at positions 2, 3 and 4. The conformation of these three hydroxyl groups in the structure is equatorial. This structure is the substrate recognition site of KP. It has been suggested that KP strictly recognizes the structures of glycerol and myo-inositol, and catalyzes the transfer reaction of a glucose residue to the hydroxyl group at position 1 in glycerol, and at position 1 or 5 in myo-inositol, corresponding to position 2 in glucose.
- 社団法人日本生物工学会の論文
- 2006-05-25
著者
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Aga Hajime
Glycoscience Institute, Hayashibara Biochemical Laboratories, Inc.
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Chaen Hiroto
Glycoscience Institute, Hayashibara Biochemical Laboratories, Inc.
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Fukuda Shigeharu
Glycoscience Institute, Hayashibara Biochemical Laboratories, Inc.
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Chaen Hiroto
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Chaen Hiroto
Glycoscience Institute Hayashibara Biochemical Laboratories Inc.
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Chaen H
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Fukuda S
Biomedical Inst. Res. Center Hayashibara Biochemical Laboratories Inc.
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Fukuda Shigeharu
Glycoscience Institute Hayashibara Biochemical Laboratories Inc.
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Kohguchi Michihiro
Fujisaki Institute Hayashibara Biochemical Laboratories Inc.
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Kurimoto M
Amase Institute Hayashibara Biochemical Laboratories Inc.
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Kibata Masayoshi
Hayashibara Biochemical Laboratories Inc.
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Watanabe H
林原生物化学研究所
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Watanabe Hikaru
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Aga H
Glycoscience Institute Hayashibara Biochemical Laboratories Inc.
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Yamamoto Takuo
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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NISHIMOTO Tomoyuki
Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
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KUBOTA Michio
Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
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Chaen Hiroto
Glycoscience Inst. Res. Center Hayashibara Biochemical Laboratories Inc.
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Kubota Michio
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Kurose Mayumi
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Mukai Kazuhisa
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Kubota M
Hayashibara Biochemical Laboratories Inc.
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Fukuda Shigeharu
Fujisaki Institute Hayashibara Biochemical Laboratories Inc.
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Kubota M
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Kurimoto M
Fujisaki Institute Hayashibam Biochemical Laboratories Inc.
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Nishimoto Tomoyuki
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Maruta K
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Fukuda Shigeharu
Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
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Yamamoto Takuo
Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
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Chaen Hiroto
Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
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