Purification and Properties of a Novel Enzyme, Trehalose Synthase, from Pimelobacter sp.R48
スポンサーリンク
概要
- 論文の詳細を見る
A novel enzyme, trehalose synthase, was purified from a cell-free extract of Pimelobacter sp. R48 to an electrophoretically homogeneous state by successive chromatographies on DEAE-Toyopearl 650, Butyl-Toyopearl 650, and Mono Q HR5/5 columns. The molecular weight of the enzyme was estimated to be 62,000 by SDS-polyacrylamide gel electrophoresis, and the enzyme had a pl of 4.6 by gel isoelectrofocusing. The enzyme catalyzed the lconversion of maltose into trehalose by intramoleculac transglucosylation. The enzyme also converted trehalose into maltose but was inactive on other saccharides. The N-terminal amino acid of the enzyme was serine. The optimum pH and temperature were pH 7.5 and 20℃, respectively. The enzyme was stable in the range of pH 6.0-9.0 and up to 30℃ for 60 min. The rate of conversion of maltose into trehalose was independent of the maltose concentration. The maximum yield of trehalose from maltose were 81.8%, 80.9%, and 76.7% at 5, 15, and 25℃, respectively. The activity was inhibited by Cu^<2+>, Hg^<2+>, Ni^<2+>, Zn^<2+>, and Tris.
- 社団法人日本農芸化学会の論文
- 1996-04-23
著者
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Nakano Masayuki
National Mito Hospital
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Chaen Hiroto
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Chaen Hiroto
Glycoscience Institute Hayashibara Biochemical Laboratories Inc.
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NISHIMOTO Tomoyuki
Hayashibara Biochemical Laboratories Inc.
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Chaen H
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Chaen Hiroto
Hayashibara Biochemical Laboratories Inc.
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Kurimoto Masashi
Hayashibara Biochemical Laboratories Inc. Fujisaki Institute
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Kurimoto Masashi
Hayashibara Biochemical Laboratories
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NARA MASATO
Shizuoka Prefectural Fisheries Experiment Station
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FUKUDA Shigeharu
Hayashibara Biochemical Laboratories
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SUGIMOTO Toshiyuki
Hayashibara Biochemical Laboratories
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TSUJISAKA Yoshio
Hayashibara Institute Corp.
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Nakano Masayuki
Hayashibara Biochemical Laboratories, Inc.
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NISHIMOTO Tomoyuki
Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
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NAKADA TETSUYA
Hayashibara Biochemical Laboratories Inc.
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Sugimoto Toshiyuki
Hayashibara Biochemical Laboratories Inc.
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Nakada T
Amase Institute Hayashibara Biochemical Laboratories Inc.
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Sugimoto T
Plant Nutrition Laboratory Faculty Of Agriculture Kobe University
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Sugimoto T
Univ. Tsukuba Tsukuba Jpn
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Kurose Mayumi
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Nakano Masatake
Thin Film Soi Group.process Engineering Dept.isobe R&d Shin-etsu Handotai Co. Ltd.
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Nakano M
National Mito Hospital
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Nishimoto Tomoyuki
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Sonoda Tomohiko
Amase Institute Hayashibara Biochemical Laboratories Inc.
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Maruta K
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Chaen Hiroto
Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
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