Cloning, Sequencing, and Expression of the Genes Encoding an Isocyclomaltooligosaccharide Glucanotransferase and an α-Amylase from a Bacillus circulans Strain
スポンサーリンク
概要
- 論文の詳細を見る
The gene for a novel glucanotransferase, isocyclomaltooligosaccharide glucanotransferase (IgtY), involved in the synthesis of a cyclomaltopentaose cyclized by an α-1,6-linkage [ICG5; cyclo-{→6)-α-D-Glcp-(1→4)-α-D-Glcp-(1→4)-α-D-Glcp-(1→4)-α-D-Glcp-(1→4)-α-D-Glcp-(1→}] from starch, was cloned from the genome of B. circulans AM7. The IgtY gene, designated igtY, consisted of 2,985 bp encoding a signal peptide of 35 amino acids and a mature protein of 960 amino acids with a calculated molecular mass of 102,071 Da. The deduced amino-acid sequence showed similarities to 6-α-maltosyltransferase, α-amylase, and cyclomaltodextrin glucanotransferase. The four conserved regions common in the α-amylase family enzymes were also found in this enzyme, indicating that this enzyme should be assigned to this family. The DNA sequence of 8,325-bp analyzed in this study contained two open reading frames (ORFs) downstream of igtY. The first ORF, designated igtZ, formed a gene cluster, igtYZ. The amino-acid sequence deduced from igtZ exhibited no similarity to any proteins with known or unknown functions. IgtZ was expressed in Escherichia coli, and the enzyme was purified. The enzyme acted on maltooligosaccharides that have a degree of polymerization (DP) of 4 or more, amylose, and soluble starch to produce glucose and maltooligosaccharides up to DP5 by a hydrolysis reaction. The enzyme (IgtZ), which has a novel amino-acid sequence, should be assigned to α-amylase. It is notable that both IgtY and IgtZ have a tandem sequence similar to a carbohydrate-binding module belonging to a family 25. These two enzymes jointly acted on raw starch, and efficiently generated ICG5.
- 社団法人 日本農芸化学会の論文
- 2006-11-23
著者
-
Chaen Hiroto
Glycoscience Institute, Hayashibara Biochemical Laboratories, Inc.
-
Fukuda Shigeharu
Glycoscience Institute, Hayashibara Biochemical Laboratories, Inc.
-
Chaen Hiroto
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
-
Chaen Hiroto
Glycoscience Institute Hayashibara Biochemical Laboratories Inc.
-
Chaen H
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
-
Fukuda S
Biomedical Inst. Res. Center Hayashibara Biochemical Laboratories Inc.
-
Fukuda Shigeharu
Glycoscience Institute Hayashibara Biochemical Laboratories Inc.
-
Kohguchi Michihiro
Fujisaki Institute Hayashibara Biochemical Laboratories Inc.
-
Kurimoto M
Amase Institute Hayashibara Biochemical Laboratories Inc.
-
Kibata Masayoshi
Hayashibara Biochemical Laboratories Inc.
-
Watanabe H
林原生物化学研究所
-
Watanabe Hikaru
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
-
NISHIMOTO Tomoyuki
Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
-
KUBOTA Michio
Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
-
Chaen Hiroto
Glycoscience Inst. Res. Center Hayashibara Biochemical Laboratories Inc.
-
Kubota Michio
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
-
Kurose Mayumi
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
-
Mukai Kazuhisa
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
-
Kubota M
Hayashibara Biochemical Laboratories Inc.
-
Fukuda Shigeharu
Fujisaki Institute Hayashibara Biochemical Laboratories Inc.
-
Kubota M
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
-
Kurimoto M
Fujisaki Institute Hayashibam Biochemical Laboratories Inc.
-
Nishimoto Tomoyuki
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
-
Maruta K
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
-
Fukuda Shigeharu
Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
-
Chaen Hiroto
Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
関連論文
- Improved yields of cyclic nigerosylnigerose from starch by pretreatment with a thermostable branching enzyme(BIOCHEMICAL ENGINEERING)
- Isolation and Identification of Antimicrobial Compounds in Brazilian Propolis
- Immuno-potentiating Effects of the Antler-shaped Fruiting Body of Ganoderma lucidum (Rokkaku-Reishi)
- Lumin, a Cyanine Dye, Enhances Interleukin 12-Dependent Interferon Gamma Production by Lipopolysaccharide-Stimulated Mouse Splenocytes
- Effect of Royal Jelly Diet on the Testicular Function of Hamsters
- Effects of Dietary Lactosucrose (4G-β-D-galactosylsucrose) on the IgE Response in Mice
- Identification of a Collagen Production-promoting Factor from an Extract of Royal Jelly and Its Possible Mechanism
- Royal Jelly Inhibits the Production of Proinflammatory Cytokines by Activated Macrophages
- Promoting Effect of Kaempferol on the Differentiation and Mineralization of Murine Pre-osteoblastic Cell Line MC3T3-E1(Food & Nutrition Science)
- Tryptanthrin Inhibits Interferon-γ Production by Peyer's Patch Lymphocytes Derived from Mice That Had Been Orally Administered Staphylococcal Enterotoxin
- Preventive Effect of Coriandrum sativum (Chinese Parsley) on Aluminum Deposition in ICR Mice
- Trehalose Ingestion Modifies Mucosal Immune Responses of the Small Intestine in Mice
- Cell differentiation and apoptosis of monocytic and promyelocytic leukemia cells (U-937 and HL-60) by tryptanthrin, an active ingredient of Polygonum tinctorium Lour
- Antibacterial action of tryptanthrin and kaempferol, isolated from the indigo plant (Polygonum tinctorium Lour.), against Helicobacter pylori-infected Mongolian gerbils
- Renal carcinogenesis induced by ferric nitrilotriacetate in mice, and protection from it by Brazilian propolis and Artepillin C
- Isolation and Identification of Compounds from Brazilian Propolis which Enhance Macrophage Spreading and Mobility
- Metastasis-Promoting Activity of a Novel Molecule, Ag 243-5, Derived from Mycoplasma, and the Complete Nucleotide Sequence
- Localization of α-Glucosidases I, II, and III in Organs of European Honeybees, Apis mellifera L., and the Origin of α-Glucosidase in Honey
- Anti-inflammatory and Immunomodulatory Properties of 2-Amino-3H-phenoxazin-3-one(Pharmacognosy)
- Purification and Substrate Specificity of Honeybee, Apis mellifera L., α-Glucosidase III(Biochemistry & Molecular Biology)
- Synthesis of Glycosyl-trehaloses by Cyclomaltodextrin Glucanotransferase through the Transglycosylation Reaction
- Effect of Dietary Cyclic Nigerosylnigerose on Intestinal Immune Functions in Mice
- Enzymatic Synthesis of a Novel Trisaccharide, Glucosyl Lactoside
- Production of Isocyclomaltopentaose from Starch Using Isocyclomaltooligosaccharide Glucanotransferase
- Cloning, Sequencing, and Expression of the Genes Encoding an Isocyclomaltooligosaccharide Glucanotransferase and an α-Amylase from a Bacillus circulans Strain
- A Novel Glucanotransferase from a Bacillus circulans Strain That Produces a Cyclomaltopentaose Cyclized by an α-1,6-Linkage
- Bioavailability of Glucosyl Hesperidin in Rats
- Suppression of Apolipoprotein B Secretion from HepG2 Cells by Glucosyl Hesperidin
- Acceptor Recognition of Kojibiose Phosphorylase from Thermoanaerobacter brockii : Syntheses of Glycosyl Glycerol and myo-Inositol(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Acceptor Specificity of Trehalose Phosphorylase from Thermoanaerobacter brockii : Production of Novel Nonreducing Trisaccharide, 6-O-α-D-Galactopyranosyl Trehalose(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Glucosyl Hesperidin Lowers Serum Triglyceride Level in Hypertriglyceridemic Subjects through the Improvement of Very Low-Density Lipoprotein Metabolic Abnormality
- Hyper Expression of Kojibiose Phosphorylase Gene and Trehalose Phosphorylase Gene from Thermoanaerobacter brockii ATCC35047 in Bacillus subtilis and Selaginose Synthesis Utilizing Two Phosphorylases(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Effects of Glucosyl Hesperidin on Serum Lipids in Hyperlipidemic Subjects : Preferential Reduction in Elevated Serum Triglyceride Level
- Gene Encoding a Trehalose Phosphorylase from Thermoanaerobacter brockii ATCC 35047(Biochemistry & Molecular Biology)
- Enzymatic Synthesis of Kojioligosaccharides Using Kojibiose Phosphorylase
- Enzymatic Synthesis of Novel Oligosaccharides from L-Sorbose, Maltose, and Sucrose Using Kojibiose Phosphorylase
- Action of a Thermostable Trehalose Synthase from Thermus aquaticus on Sucrose
- Synthesis by an α-Glucosidase of Glycosyl-trehaloses with an Isomaltosyl Residue
- Purification and Characterization of a Thermostable Trehalose Synthase from Thermus aquaticus
- Purification and Properties of a Novel Enzyme, Trehalose Synthase, from Pimelobacter sp.R48
- Purification and Characterization of Thermostable Maltooligosyl Trehalose Trehalohydrolase from the Thermoacidophilic Archaebacterium Sulfolobus acidocaldarius
- Purification and Characterization of Cyclic Maltosyl-(1→6)-Maltose Hydrolase and α-Glucosidase from an Arthrobacter globiformis Strain
- Antimicrobial activity of Polygonum tinctorium Lour : extract against oral pathogenic bacteria
- 6-α-Glucosyltransferase and 3-α-Isomaltosyltransferase from Bacillus globisporus N75
- Cloning and Sequencing of Trehalose Biosynthesis Genes from Rhizobium sp. M-11
- Purification and Characterization of Thermostable Maltooligosyl Trehalose Synthase from the Thermoacidophilic Archaebacterium Sulfolobus acidocaldarius
- Purification and Characterization of a Novel Enzyme, Maltooligosyl Trehalose Trehalohydrolase, from Arthrobacter sp. Q36
- Purification and Properties of a Novel Enzyme, Maltooligosyl Trehalose Synthase, from Arthrobacter sp. Q36
- Formation of Trehalose from Maltooligosaccharides by a Novel Enzymatic System
- Purification and Characterization of Two Forms of Maltotetraose-forming Amylase from Pseudomonas stutzeri(Biological Chemistry)
- The Effect of AgK114 on Wound Healing(Highlighted paper selected by Editor-in-chief,Molecular and Cell Biology)
- Distribution of a Novel Protein AgK114 Expression in the Normal Tissues of Adult Mice : Dual Expression of AgK114 and Growth Hormone in Anterior Pituitary Cells(Endocrinology)
- Suppressive Effect of Trehalose on Acrylamide Formation from Asparagine and Reducing Saccharides
- Existence of a Novel Enzyme Converting Maltose into Trehalose
- Autocrine Interferon-β Stimulation Augments Nitric Oxide Production by Mouse Macrophage J774A.1 Cells Infected with Herpes Simplex Virus Type 1
- Enhancement of Thermostability of Kojibiose Phosphorylase from Thermoanaerobacter brockii ATCC35047 by Random Mutagenesis(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Interaction between Trehalose and Alkaline-Earth Metal Ions
- Cyclic Tetrasaccharide-Synthesizing Enzymes from Arthrobacter globiformis A19
- Enzymatic Synthesis of Glycosyl Cyclic Tetrasaccharide with 6-α-Glucosyltransferase and 3-α-Isomaltosyltransferase(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Cloning and Sequencing of Kojibiose Phosphorylase Gene from Thermoanaerobacter brockii ATCC35047(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Synthesis of 3-O-β-N-Acetylglucosaminyl Cyclic Tetrasaccharide through a Lysozyme-catalyzed Transfer Reaction(Biochemistry & Molecular Biology)
- Transglycosylation of Glycosyl Residues to Cyclic Tetrasaccharide by Bacillus stearothermophilus Cyclomaltodextrin Glucanotransferase Using Cyclomaltodextrin as the Glycosyl Donor(Biochemistry & Molecular Biology)
- Production of Cyclic Tetrasaccharide from Starch Using a Novel Enzyme System from Bacillus glob isporus C11
- Substrate Specificity and Subsite Affinities of Honeybee α-Glucosidase II
- Carbohydrate Structures of the Glycoprotein Allergen Cry j I from Japanese Cedar (Cryptomeria japonica) Pollen
- Enzymatic Properties of Recombinant Kojibiose Phosphorylase from Caldicellulosiruptor saccharolyticus ATCC43494
- Inhibitory Effects of 2-Amino-3H-phenoxazin-3-one on the Melanogenesis of Murine B16 Melanoma Cell Line
- Purification and Characterization of Glucosyltransferase and Glucanotransferase Involved in the Production of Cyclic Tetrasaccharide in Bacillus globisporus C11(Biochemistry & Molecular Biology)
- Cloning and Sequencing of the Genes Encoding Cyclic Tetrasaccharidesynthesizing Enzymes from Bacillus globisporus C11(Biochemistry & Molecular Biology)
- Cloning and Sequencing of the β-Fructofuranosidase gene from Bacillus sp.
- Trehalose-producing Operon treYZ from Arthrobacter ramosus S34
- Purification and Characterization of Cyclic Maltosyl-(1→6)-Maltose Hydrolase and α-Glucosidase from an Arthrobacter globiformis Strain
- Identification of the Novel Membrane-Associated Protein AgK114 on Hamster Keratinocytes Recognized by a Monoclonal Antibody K114(Biochemistry/Molecular Biology)
- Lactosucrose Inhibits Body Fat Accumulation in Rats by Decreasing Intestinal Lipid Absorption
- Effects of Dietary Lactosucrose (4^G-β-D-galactosylsucrose) on the IgE Response in Mice
- Prolonged Stability of Natural Human Interferon Alpha (IFN-α) Lozenges Using Anhydrous Maltose as an Excipient
- Cloning, Sequencing and Expression of the Genes Encoding Cyclic α-Maltosyl-(1→6)-maltose Hydrolase and α-Glucosidase from an Arthrobacter globiformis Strain
- Antiviral Effect of Polygonum tinctorium Lour. Extracts on Virus-Infected Cells
- Enzymatic Properties of Recombinant Kojibiose Phosphorylase from Caldicellulosiruptor saccharolyticus ATCC43494
- Preventive Effect of Coriandrum sativum (Chinese Parsley) on Aluminum Deposition in ICR Mice
- Isolation and Identification of Anti-Helicobacter pylori Compounds from Polygonum tinctorium Lour.
- Anti-Helicobacter pylori Compounds in Brazilian Propolis
- Purification and Characterization of Highly Branched α-Glucan–Producing Enzymes from Paenibacillus sp. PP710
- Purification and Characterization of Highly Branched α-Glucan-Producing Enzymes from Paenibacillus sp. PP710