Transglycosylation of Glycosyl Residues to Cyclic Tetrasaccharide by Bacillus stearothermophilus Cyclomaltodextrin Glucanotransferase Using Cyclomaltodextrin as the Glycosyl Donor(Biochemistry & Molecular Biology)

概要

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Cyclomaltodextrin glucanotransferase (EC 2. 4. 1. 19, abbreviated as CGTase) derived from Bacillus stearothermophilus produced a series of transfer products from a mixture of cyclomaltohexaose and cyclic tetrasaccharide (cyclo {→6) -α-D-Glcp- (1→3) -α-D-Glcp- (1→6) -α-D-Glcp- (1→3) -α-D-Glcp- (1→}, CTS). Of the transfer products, only two components, saccharides A and D, remained and accumulated after digestion with glucoamylase. The total combined yield of the saccharides reached 63.4% of total sugars, and enzymatic and instrumental analyses revealed the structures of both saccharides. Saccharide A was identified as 4-mono-O-α-glucosyl-CTS, {→6) - [α-D-Glcp- (1→4)] -α-D-Glcp- (1→3) -α-D-Glcp- (1→6)-α-D-Glcp- (1→3) -α-D-Glcp- (1→}, and sachharide D was 4, 4'-di-O-α-glucosyl-CTS, {→6) - [α-D-Glcp- (1→4)] -α-D-Glcp- (1→3) -α-D-Glcp- (1→6) - [α-D-Glcp- (1→4)] -α-D-Glcp- (1→3) -α-D-Glcp- (1→}. These structures led us to conclude that the glycosyltransfer catalyzed by CGTase was specific to the C4-OH of the 6-linked glucopyranosyl residues in CTS.
社団法人日本農芸化学会の論文
2003-05-23