Purification and Characterization of a Thermostable Trehalose Synthase from Thermus aquaticus
スポンサーリンク
概要
- 論文の詳細を見る
Thermostable trehalose synthase, which catalyzes the conversion of maltose into trehalose by intramolecular transglucosylation, was purified from a cell-free extract of the thermophilic bacterium Thermus aquaticus ATCC 33923 to an electrophoretically homogeneity by successive column chromatographies. The purified enzyme had a molecular weight of 105,000 by SDS-polyacrylamide gel electrophoresis and a pI of 4.6 by gel isoelectrofocusing. The N-terminal amino acid of the enzyme was methionine. The optimum pH and temperature were pH 6.5 and 65℃, respectively. The enzyme was stable from pH 5.5 to 9.5 and up to 80℃ for 60 min. The trehalose synthase from Thermus aquaticus is more thermoactive and thermostable than that from Pimelobacter sp. R48. The yield of trehalose from maltose by the enzyme was independent of the substrate concentration, and tended to increase at lower temperatures. The maximum yield of trehalose from maltose by the enzyme reached 80-82% at 30-40℃. The activity was inhibited by Cu^<2+>, Hg^<2+>, Zn^<2+>, and Tris.
- 社団法人日本農芸化学会の論文
- 1996-05-23
著者
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Chaen Hiroto
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Chaen Hiroto
Glycoscience Institute Hayashibara Biochemical Laboratories Inc.
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NISHIMOTO Tomoyuki
Hayashibara Biochemical Laboratories Inc.
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Chaen H
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Chaen Hiroto
Hayashibara Biochemical Laboratories Inc.
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Kurimoto Masashi
Hayashibara Biochemical Laboratories Inc. Fujisaki Institute
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Kurimoto Masashi
Hayashibara Biochemical Laboratories
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FUKUDA Shigeharu
Hayashibara Biochemical Laboratories
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SUGIMOTO Toshiyuki
Hayashibara Biochemical Laboratories
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TSUJISAKA Yoshio
Hayashibara Institute Corp.
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NISHIMOTO Tomoyuki
Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
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NAKADA TETSUYA
Hayashibara Biochemical Laboratories Inc.
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Sugimoto Toshiyuki
Hayashibara Biochemical Laboratories Inc.
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Nakada T
Amase Institute Hayashibara Biochemical Laboratories Inc.
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Sonoda Tadamichi
The Institute Of Physical And Chemical Research (riken):(present Address) Riken Vitamin Co. Ltd.
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Kurose Mayumi
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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SONODA TOMOHIKO
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
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Tsujisaka Y
Biomedical Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Nishimoto Tomoyuki
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Sonoda Tomohiko
Amase Institute Hayashibara Biochemical Laboratories Inc.
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Maruta K
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Chaen Hiroto
Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
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