Production of Cyclic Tetrasaccharide from Starch Using a Novel Enzyme System from Bacillus glob isporus C11
スポンサーリンク
概要
- 論文の詳細を見る
Production of cyclo{★6)-★-D-Glcp-(1★3)-★-D-Glcp-(1★6)-★-D-Glcp-(1★3)-★-D-Glcp-(1★} (CTS, cyclic tetrasaccharide) from starch was attempted using 1,6-★-glucosyltransferase (6GT) and 1,3-★-isomaltosyltransferase (IMT) from Bacillus globisporus C11. The optimal conditions for production from partially hydrolyzed starch were as follows : substrate concentration, 3%; pH6-7; temperature, 30★; 6GT, 1 unit/g-dry solid (DS); IMT, 10 units/g-DS. The production of CTS was demonstrated and 544g of CTS hydrate crystal powders were obtained from 3500g of partially hydrolyzed starch. Two major by-products were also isolated from the reaction mixture and identified as the branched derivatives of CTSs, 4-O-★-D-glucopyranosyl-CTS and 3-O-★-isomaltosyl-CTS.
- 社団法人日本生物工学会の論文
- 2002-10-25
著者
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Aga Hajime
Amase Institute, Hayashibara Biochemical Laboratories Inc.
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Kurimoto Masashi
Amase Institute, Hayashibara Biochemical Laboratories Inc.
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WATANABE Hikaru
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
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FUKUDA Shigeharu
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
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Fukuda S
Biomedical Inst. Res. Center Hayashibara Biochemical Laboratories Inc.
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Fukuda Shigeharu
Glycoscience Institute Hayashibara Biochemical Laboratories Inc.
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Kohguchi Michihiro
Fujisaki Institute Hayashibara Biochemical Laboratories Inc.
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Kurimoto M
Amase Institute Hayashibara Biochemical Laboratories Inc.
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Watanabe H
林原生物化学研究所
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Watanabe Hikaru
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Aga H
Glycoscience Institute Hayashibara Biochemical Laboratories Inc.
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Aga Hajime
Amase Institute Hayashibara Biochemical Laboratories Inc.
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NISHIMOTO Tomoyuki
Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
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Nishimoto Tomoyuki
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
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Kubota Michio
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
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Tsujisaka Yoshio
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
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Sugimoto Toshiyuki
Hayashibara Biochemical Laboratories Inc.
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Taniguchi Yoshifumi
Biomedical Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.
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Sonoda Tadamichi
The Institute Of Physical And Chemical Research (riken):(present Address) Riken Vitamin Co. Ltd.
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Kurose Mayumi
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Mukai Kazuhisa
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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HIGASHIYAMA TAKANOBU
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
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SONODA TOMOHIKO
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
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Tsujisaka Y
Biomedical Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Nishimoto Tomoyuki
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Sonoda Tomohiko
Amase Institute Hayashibara Biochemical Laboratories Inc.
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Maruta K
Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Taniguchi Yoshifumi
Biomedical Institute Research Center Hayashibara Biochemical Laboratories Inc.
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Higashiyama Takanobu
Amase Institute Hayashibara Biochemical Laboratories Inc.
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