Production of Cyclic Tetrasaccharide from Starch Using a Novel Enzyme System from Bacillus glob isporus C11

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概要

• 論文の詳細を見る

• Production of cyclo{★6)-★-D-Glcp-(1★3)-★-D-Glcp-(1★6)-★-D-Glcp-(1★3)-★-D-Glcp-(1★} (CTS, cyclic tetrasaccharide) from starch was attempted using 1,6-★-glucosyltransferase (6GT) and 1,3-★-isomaltosyltransferase (IMT) from Bacillus globisporus C11. The optimal conditions for production from partially hydrolyzed starch were as follows : substrate concentration, 3%; pH6-7; temperature, 30★; 6GT, 1 unit/g-dry solid (DS); IMT, 10 units/g-DS. The production of CTS was demonstrated and 544g of CTS hydrate crystal powders were obtained from 3500g of partially hydrolyzed starch. Two major by-products were also isolated from the reaction mixture and identified as the branched derivatives of CTSs, 4-O-★-D-glucopyranosyl-CTS and 3-O-★-isomaltosyl-CTS.

• 社団法人日本生物工学会の論文
• 2002-10-25

著者

• Aga Hajime

  Amase Institute, Hayashibara Biochemical Laboratories Inc.

• Kurimoto Masashi

  Amase Institute, Hayashibara Biochemical Laboratories Inc.

• WATANABE Hikaru

  Amase Institute, Hayashibara Biochemical Laboratories, Inc.

• FUKUDA Shigeharu

  Amase Institute, Hayashibara Biochemical Laboratories, Inc.

• Fukuda S

  Biomedical Inst. Res. Center Hayashibara Biochemical Laboratories Inc.

• Fukuda Shigeharu

  Glycoscience Institute Hayashibara Biochemical Laboratories Inc.

• Kohguchi Michihiro

  Fujisaki Institute Hayashibara Biochemical Laboratories Inc.

• Kurimoto M

  Amase Institute Hayashibara Biochemical Laboratories Inc.

• Watanabe H

  林原生物化学研究所

• Watanabe Hikaru

  Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.

• Aga H

  Glycoscience Institute Hayashibara Biochemical Laboratories Inc.

• Aga Hajime

  Amase Institute Hayashibara Biochemical Laboratories Inc.

• NISHIMOTO Tomoyuki

  Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.

• Nishimoto Tomoyuki

  Amase Institute, Hayashibara Biochemical Laboratories, Inc.

• Kubota Michio

  Amase Institute, Hayashibara Biochemical Laboratories, Inc.

• Tsujisaka Yoshio

  Amase Institute, Hayashibara Biochemical Laboratories, Inc.

• Sugimoto Toshiyuki

  Hayashibara Biochemical Laboratories Inc.

• Taniguchi Yoshifumi

  Biomedical Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.

• Sonoda Tadamichi

  The Institute Of Physical And Chemical Research (riken):(present Address) Riken Vitamin Co. Ltd.

• Kurose Mayumi

  Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.

• Mukai Kazuhisa

  Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.

• HIGASHIYAMA TAKANOBU

  Amase Institute, Hayashibara Biochemical Laboratories, Inc.

• SONODA TOMOHIKO

  Amase Institute, Hayashibara Biochemical Laboratories, Inc.

• Tsujisaka Y

  Biomedical Institute Research Center Hayashibara Biochemical Laboratories Inc.

• Nishimoto Tomoyuki

  Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.

• Sonoda Tomohiko

  Amase Institute Hayashibara Biochemical Laboratories Inc.

• Maruta K

  Glycoscience Institute Research Center Hayashibara Biochemical Laboratories Inc.

• Taniguchi Yoshifumi

  Biomedical Institute Research Center Hayashibara Biochemical Laboratories Inc.

• Higashiyama Takanobu

  Amase Institute Hayashibara Biochemical Laboratories Inc.

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