Synthesis of 2-O-α-D-Glucopyranosyl L-Ascorbic Acid by Cyclomaltodextrin Glucanotransferase from Bacillus stearothermophilus(Biological Chemistry)
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概要
- 論文の詳細を見る
Cyclomaltodextrin glucanotransferase (CGTase) [EC 2.4.1.19] was found to catalyze the transglycosylation from α-cyclodextrin (α-CD) to L-ascorbic acid (AA). A main product formed by this reaction was identified as 2-O-α-D-glucopyranosyl L-ascorbic acid (AA-2G) with the enzymatic hydrolysis and ultraviolet absorption spectra using standard AA-2G. A series of maltooligosaccharide substituted 2-O-derivatives of AA were also detected by HPLC. These were thoroughly hydrolized to AA-2G and glucose by treatment with glucoamylase [EC 3.2.1.3]. A large amount of AA-2G was prepared with 500g of AA and 1000g of α-CD. Two hundred and fifty grams of purified AA-2G, the content of which was 97%, was obtained through enzyme reactions and purification with HPLC using an ion-exchange resin.
- 社団法人日本農芸化学会の論文
- 1991-07-23
著者
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Aga Hajime
Amase Institute, Hayashibara Biochemical Laboratories Inc.
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Aga Hajime
Amase Institute Hayashibara Biochemical Laboratories Inc.
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YAMAMOTO Itaru
Department of Radiology, Kanazawa Medical University
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Yamamoto Itaru
Department Of Immunochemistry Faculty Of Pharmaceutical Sciences Okayama University
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Yamamoto Itaru
Department Of Immunochemistry Faculty Of Pharmaceutical Science Okayama University
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Sakai Shuzo
Amase Institute Hayashibara Biochemical Laboratories Inc.
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YONEYAMA Masaru
Amase Institute, Hayashibara Biochemical Laboratories, Inc.
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Yoneyama Masaru
Amase Institute Hayashibara Biochemical Laboratories Inc.
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Yoneyama Masaru
Amase Institute Hayashibara Biochemical Laboratories
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