Formation of a Stable Ascorbic Acid 2-Glucoside by Specific Transglucosylation with Rice Seed α-Glucosidase(Biological Chemistry)
スポンサーリンク
概要
- 論文の詳細を見る
The enzymatic transglucosylation to synthesize a chemically stable form of L-ascorbic acid (AA) was further investigated by using commercially available enzymes. Among various glycosidases examined, only rice seed α-glucosidase could produce a nonreducing and stable glucoside of AA, which was identified as 2-O-α-D-glucopyranosyl-L-ascorbic acid (AA-2G). The enzyme showed the same regioselective transglucosylase activity as rat intestinal α-glucosidase that had been demonstrated by us to be effective in this reaction, although these two enzymes had different pH optima for maltose hydrolysis. The substrate specificity of rice α-glucosidase for AA-2G formation was considerably different from that of rat α-glucosidase. However, both α-glucosidases had high specificity for the α-1,4-glucosidic linkage but not α-1,6, suggesting that they can catalyze a preferential transglucosylation to the 2-position but not to the 6-position of AA. Thus, these results allows us to produce a sufficient amount of AA-2G with rice seed α-glucosidase for its application to medicinal and other uses.
- 社団法人日本農芸化学会の論文
- 1990-07-23
著者
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Goto Kazuko
Department Of Immunochemistry Faculty Of Pharmaceutical Sciences Okayama University
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Muto Norio
Fac. Of Life And Environmental Sci. Prefectural Univ. Of Hiroshima
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YAMAMOTO Itaru
Department of Radiology, Kanazawa Medical University
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Muto N
Okayama Univ. Okayama
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Yamamoto Itaru
Okayama Univ. Okayama Jpn
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Yamamoto I
Okayama Univ. Okayama Jpn
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SUGA Sadaharu
Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University
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MUTO Norio
Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University
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FUJII Kazuko
Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University
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Muto Norio
Department Of Immunochemistry Faculty Of Pharmaceutical Sciences Okayama University
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Yamamoto Itaru
Department Of Immunochemistry Faculty Of Pharmaceutical Science Okayama University
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Fujii Kazuko
Department Of Immunochemistry Faculty Of Pharmaceutical Sciences Okayama University
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Suga S
Okayama Univ. Okayama
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Suga Sadaharu
Department Of Immunochemistry Faculty Of Pharmaceutical Sciences Okayama University
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Muto N
Graduate School Of Comprehensive Scientific Research Program In Biological System Sciences Prefectur
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