Essential Role of Ascorbic Acid in Neural Differentiation and Development : High Levels of Ascorbic Acid 2-Glucoside Effectively Enhance Nerve Growth Factor-Induced Neurite Formation and Elongation in PC12 Cells

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The aim of this study was to investigate the role of ascorbic acid (AsA) in neural differentiation induced by nerve growth factor (NGF) in PC12 cells, a neural precursor cell line. Cells were cultivated in the presence of L-ascorbic acid-2-O-a-D-glucopyranoside (AA-2G) and evaluated for potentiation of neural differentiation induced by a small amount of NGF. AA-2G enhanced the proportion of neurite-bearing cells in a concentration-dependent manner (<1mM). When cultivated with relatively high concentrations of AA-2G (1-5mM), the cells tended to extend neurites further and to form a neuron-like network. The resultant number of neurite-bearing cells was apparently increased by the addition of AA-2G, with a concurrent decrease in the number of total cells in the same culture dish. These results suggest that AsA in the brain not only promotes NGF-induced differentiation of neural precursor cells but also participates in the development of neural network-forming cells.
社団法人日本薬学会の論文
2008-02-01