The use of .BETA.-cyclodextrin-containing culture medium for in vitro evaluation of immunomodulating agents.

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概要

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• We have devised a new culture medium that is made of RPMI-1640 medium, 500 μg/ml β-cyclodextrin (β-CD) and 1% fetal calf serum (FCS) (β-CD medium). Murine lymphocytes stimulated with sheep erythrocytes in vitro developed antibody-forming cells in β-CD medium as efficiently as in 10% FCS-containing RPMI-1640 medium (10F medium). Immunostimulating agents (SA96 and levamisole) and immunosuppressive agents (hydrocortisone and D-penicillamine) showed similar immunomodulating effects on the antibody responses in both media. In addition, the enhancing effect of levamisole varied drastically depending on the lot of FCS used in 1OF medium, but a clear enhancement was always observed in β-CD medium containing any lot of FCS tested.

• 公益社団法人 日本薬理学会の論文

著者

• Ohmori Hitoshi

  Department Of Bioscience And Biotechnology Graduate School Of Natural Science And Technology Okayama

• Yamamoto Itaru

  Department Of Immunochemistry Faculty Of Pharmaceutical Science Okayama University

• Ienaga Midori

  Department Of Immunochemistry Faculty Of Pharmaceutical Sciences Okayama University

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