Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line(CELL AND TISSUE ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
A hypermutating B cell line DT40 is useful for screening antibodies and improving affinity of the selected antibodies in vitro. To perform affinity maturation efficiently, we generated an engineered DT40 line whose immunoglobulin mutation pattern can be transformed from gene conversion into point mutation by conditional suppression of XRCC3 expression.
著者
-
Kajita Masamichi
Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okaya
-
Magari Masaki
Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okaya
-
Todo Kagefumi
Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okaya
-
Kanayama Naoki
Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okaya
-
Ohmori Hitoshi
Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okaya
-
Kanayama Naoki
Department Of Bioscience And Biotechnology Graduate School Of Natural Science And Technology Okayama
-
Todo Kagefumi
Department Of Bioscience And Biotechnology Graduate School Of Natural Science And Technology Okayama
-
Magari Masaki
Department Of Bioscience And Biotechnology Graduate School Of Natural Science And Technology Okayama
-
Ohmori Hitoshi
Department Of Bioscience And Biotechnology Graduate School Of Natural Science And Technology Okayama
-
Kajita Masamichi
Department Of Bioscience And Biotechnology Graduate School Of Natural Science And Technology Okayama
関連論文
- Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line(CELL AND TISSUE ENGINEERING)
- Efficient affinity maturation of antibodies in an engineered chicken B cell line DT40-SW by increasing point mutation
- The Vanstone-Zuccherato Schemes Revisited(Information Security)
- Efficient affinity maturation of antibodies in an engineered chicken B cell line DT40-SW by increasing point mutation(CELL AND TISSUE ENGINEERING)
- Optimised Versions of the Ate and Twisted Ate Pairings
- Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line
- Enhancement of antibody production from a chicken B cell line DT40 by reducing Pax5 expression(MEDICAL BIOTECHNOLOGY)
- Novel In Vitro Screening System for Monoclonal Antibodies Using Hypermutating Chicken B Cell Library(METHOD)
- ACTIVATION OF MURINE LYMPHOCYTES BY 2-MERCAPTOETHANOL AND RELATED THIOL COMPOUNDS AND ITS MECHANISM. II. EFFECT OF THIOL COMPOUNDS ON THE VIABILITY OF THE LYMPHOCYTES UNDER A CYSTINE-DEFICIENT CULTURE CONDITION
- IN VITRO GENERATION OF ANTIGEN-SPECIFIC SUPPRESSOR T CELL ACTIVITY. CULTURE CONDITIONS FOR ABROGATING THE INDUCTION OF NONSPECIFIC SUPPRESSOR T CELLS
- Optimised Versions of the Ate and Twisted Ate Pairings
- Enhancement of Transgene Expression in Mammalian Cell Line by a Δ^7-Prostaglandin A_1 Analogue
- High mobility group box-1-inducible melanoma inhibitory activity is associated with nodal metastasis and lymphangiogenesis in oral squamous cell carcinoma
- Small Bowel Obstruction Caused by Anisakiasis of the Small Intestine : Report of a Case
- Transmesosigmoid Hernia : Report of a Case
- THE USE OF β-CYCLODEXTRIN IN LYMPHOCYTE CULTURE. INDUCTION OF THE PRIMARY ANTIBODY RESPONSE IN VITRO IN CALF SERUM- OR NEWBORN BOVINE SERUM-CONTAINING CULTURE MEDIUM
- DEGRADATION OF HISTAMINE IN THE PRESENCE OF ASCORBIC ACID AND Cu^ ION : INVOLVEMENT OF HYDROGEN PEROXIDE
- Genomic Structures and Chromosomal Location of p91, a Novel Murine Regulatory Receptor Family^1
- 5-Azacytidine Enhances Transient Expression of a Transfected Gene in Cultured Mammalian Cells
- Enhancement of Expression of the Introduced Gene by 5-Azacytidine in Mammalian Cell Lines
- Methionine-enkephalin secreted by human colorectal cancer cells suppresses T lymphocytes
- Characterization of ASK Mice, a Strain Highly Sensitive to Anaphylactic Shock
- Suppression of in Vitro Cellular Immune Response by Nitrogen-Containing Terpene Alcohol Derivatives
- Enhancement of Expression of Introduced β-Galactosidase Gene by 2-Aminopurine in Mammalian Cell Lines
- Analysis of Baby-Step Giant-Step Algorithms for Non-uniform Distributions (Fundamental) (Cryptography and Information Security)
- Generating Secure Genus Two Hyperelliptic Curves Using Elkies' Point Counting Algorithm
- EFFECT OF N-(2-CARBOXYPHENYL)-4-CHLOROANTHRANILIC ACID DISODIUM SALT (CCA) ON THE INDUCTION OF HELPER AND SUPPRESSOR T CELLS IN VITRO AND IN VIVO
- Production of an Immunosuppressive Polysaccharide, AZ9, in the Culture of Klebsiella oxytoca Strain TNM3
- Search for Immunosuppressive Substances Produced in the Culture of Enteric Bacteria Isolated from Marine Fish
- Subconjunctival Immunization of Mice for Inducing IgE Antibody Response in Parotic Lymph Node
- Scalar Multiplication on Pairing Friendly Elliptic Curves
- Augmentation of the antibody response by lipoic acid in mice. I. Analysis of the mode of action in an in vitro culture system.
- A Note on the Pairing Computation Using Normalized Miller Functions
- Advanced glycation end products (AGE) induce the receptor for AGE in the colonic mucosa of azoxymethane-injected Fischer 344 rats fed with a high-linoleic acid and high-glucose diet
- Phorbol Ester-Induced Reversible Inactivation of Cytotoxic T Cell Function: Correlation with Down-Regulation of Protein Kinase C Activity.
- Implementation of an Elliptic Curve Scalar Multiplication Method Using Division Polynomials
- Demonstration of the Requirements for Cholesterol or Low Density Lipoprotein in the Antibody Response of Murine Lymphocytes in Serum-free Culture
- The use of .BETA.-cyclodextrin-containing culture medium for in vitro evaluation of immunomodulating agents.
- Augmentation of in vitro antibody response by disulfide compounds. I. Comparison between intermolecular and intramolecular disulfides.
- Augmentation of the antibody response by lipoic acid in mice. II Restoration of the antibody response in immunosuppressed mice.