Enhancement of antibody production from a chicken B cell line DT40 by reducing Pax5 expression(MEDICAL BIOTECHNOLOGY)
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概要
- 論文の詳細を見る
We developed a novel in vitro antibody (Ab) generation system using a hypermutating chicken B cell line (DT40-SW). We suppressed the expression of the Pax5 transcription factor by targeted disruption of the gene to increase Ab production in isolated clones and produce the desired Abs. This single genetic manipulation resulted in a significant enhancement of Ab production without significantly affecting maximum cell density.
- 社団法人日本生物工学会の論文
著者
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Magari Masaki
Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okaya
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Todo Kagefumi
Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okaya
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Kanayama Naoki
Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okaya
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Ohmori Hitoshi
Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okaya
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Ikeda Mika
Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okaya
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Kanayama Naoki
Department Of Bioscience And Biotechnology Graduate School Of Natural Science And Technology Okayama
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Ikeda Mika
Department Of Bioscience And Biotechnology Graduate School Of Natural Science And Technology Okayama
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Ikeda Mika
岡山大学 大学院自然科学研究科生物機能工学科
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Todo Kagefumi
Department Of Bioscience And Biotechnology Graduate School Of Natural Science And Technology Okayama
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Aya Takahiro
Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okaya
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Aya Takahiro
Department Of Bioscience And Biotechnology Graduate School Of Natural Science And Technology Okayama
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Aya Takahiro
岡山大学 大学院自然科学研究科生物機能工学科
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Magari Masaki
Department Of Bioscience And Biotechnology Graduate School Of Natural Science And Technology Okayama
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Ohmori Hitoshi
Department Of Bioscience And Biotechnology Graduate School Of Natural Science And Technology Okayama
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