Enhancement of Expression of Introduced β-Galactosidase Gene by 2-Aminopurine in Mammalian Cell Lines
スポンサーリンク
概要
- 論文の詳細を見る
We transfected a mouse myeloma cell line, P3/NS1-Ag4-1 (NS-1), and a chinese hamster ovary cell line, CHO-K1,with the β-galactosidase (β-Gal) gene of Escherichia coli, and isolated stable transformants, designated as NS-1Z/gpt and CHO-Z/neo, respectively. When these cells were incubated with 5-10 mM 2-aminopurine (2-AP) for 12-24 h, the specific and the total activity of β-Gal was enhances 2- to 4-fold and 1.5-to 2-fold, respectively. It was confirmed in immunotitration experiments that the enhancement of β-Gal activity was due to the increase of the enzyme protein. Northern blot analysis revealed that 2-AP augmented the expression of β-Gal mRNA. On the other hand, 2-AP did not significantly affect the expression of an endogenous gene like lactate dehydrogenase or β-actin.
- 社団法人日本生物工学会の論文
- 1993-08-25
著者
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Ohmori Hitoshi
Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okaya
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Ohmori H
Riken
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Ohmori H
Okayama Univ. Okayama Jpn
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Ohmori Hitoshi
Department Of Bioscience And Biotechnology Graduate School Of Natural Science And Technology Okayama
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Ohmori Hitoshi
The Institute Of Physical And Chemical Research
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Ohmori H
Inst. Physical And Chemical Res.(riken) Saitama‐pref. Jpn
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TAKAI TOSHIYUKI
Department of Biotechnology, Faculty of Engineering Okayama University
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HIKIDA MASAKI
Department of Biotechnology, Faculty of Engineering Okayama University
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Hikida M
Okayama Univ. Okayama Jpn
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Hikida Masaki
Department Of Biotechnology Faculty Of Engineering Okayama University
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TANIGAWA TAKAHIRO
Department of Biotechnology, Faculty of Engineering, Okayama University
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Tanigawa Takahiro
Department Of Biotechnology Faculty Of Engineering Okayama University
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Takai Toshiyuki
Department Of Biotechnology Faculty Of Engineering Okayama University
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