Enhancement of Expression of the Introduced Gene by 5-Azacytidine in Mammalian Cell Lines

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概要

• 論文の詳細を見る

• We transfected a mouse myeloma cell line, P3/NS1-Ag4-1 (NS-1), and a Chinese hamster ovary cell line, CHO-K1 with the β-galactosidase (β-Gal) gene of Escherichia coli, and isolated stable transformants designated as NS-1Z/gpt and CHO-Z/neo, respectively. When NS-1Z/gpt cells were incubated with 5-20 μM 5-azacyti-dine (5-azaC), the specific and total activity of β-Gal were enhanced 2- to 3-fold and 1.5- to 2-fold, respectively. In CHO-Z/neo cells, similar treatment reslted in a 3- to 5-fold increase in the specific β-Gal activity and about a 2-fold enhancement in total enzyme activity. The growth of both cell lines was inhibited by more than 80% in the presence of 10 μM 5-azaC. It was confirmed in immunotitration experiments that the enhancement of β-Gal activities was due to an increase in the enzyme protein. Northern blot analysis revealed that 5-azaC-treatment resulted in the enhanced expression of β-Gal mRNA. 5-AzaC also enhanced the production of human interleukin 2 (IL2) from CHO cells that were tranfected with the IL2 gene. On the other hand, 5-azaC did not significantly affect the expression of an endogenous gene like lactate dehydrogenase or β-actin. These results suggest that 5-azaC is a useful agent for up-regulating the expression of introduced genes.

• 社団法人日本生物工学会の論文
• 1993-04-25

著者

• Ohmori Hitoshi

  Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okaya

• Ohmori H

  Riken

• Ohmori H

  Okayama Univ. Okayama Jpn

• Ohmori Hitoshi

  Department Of Bioscience And Biotechnology Graduate School Of Natural Science And Technology Okayama

• Ohmori Hitoshi

  The Institute Of Physical And Chemical Research

• Yasuda T

  Graduate School Of Science And Technology Kobe University

• Ohmori H

  Inst. Physical And Chemical Res.(riken) Saitama‐pref. Jpn

• TAKAI TOSHIYUKI

  Department of Biotechnology, Faculty of Engineering Okayama University

• HIKIDA MASAKI

  Department of Biotechnology, Faculty of Engineering Okayama University

• Yasuda Tatsuji

  Institute Of Cellular And Molecular Biology Okayama University Medical School

• Hikida M

  Okayama Univ. Okayama Jpn

• Hikida Masaki

  Department Of Biotechnology Faculty Of Engineering Okayama University

• TANIGAWA TAKAHIRO

  Department of Biotechnology, Faculty of Engineering, Okayama University

• YASUDA TATSUJI

  Cellular and Molecular Biology, Okayama University Medical School

• Tanigawa Takahiro

  Department Of Biotechnology Faculty Of Engineering Okayama University

• Takai Toshiyuki

  Department Of Biotechnology Faculty Of Engineering Okayama University

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