Immobilization of Candida Cells Showing Mycelium-Like Shapes in Porous Polyvinyl Formal Resin and Their Applications
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概要
- 論文の詳細を見る
Candida rugosa ATCC14830 cells have high tendency to make flocculent masses by entanglement of the mycelium-like shaped cells and can be well immobilized in porous polyvinyl formal (PVF) resin. Moreover, Candida lipolytica cells, which became to show a mycelium-like shape by mutation, were also immobilized in PVF resin.These C. rugosa and mutant C. lipolytica cells were immobilized in PVF resins and cultured by the draw-fill method 2–3 times. These C. rugosa and C. lipolytica cells could decompose L-lactic acid and produce bio-surfactant liposan, respectively. Because high cell densities could be attained in immobilized systems, the total productivity in repeated-batch operations with immobilized cells was higher than those in batch operations with free cells. Moreover, the decomposed amount of L-lactic acid did not decrease during the repeated-batch operations, because the number of immobilized cells in PVF resin was kept almost constant.
- 社団法人 化学工学会の論文
- 2003-02-01
著者
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INOUE Yoko
Department of Preventive Medicine and Public Health, Kitasato University School of Medicine
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Inoue Y
Keio Univ. Kanagawa Jpn
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Yasuda T
Graduate School Of Science And Technology Kobe University
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Shiomi Naofumi
Department of Biosphere Sciences, Kobe College
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Tomioka Kanji
Department Of Biochemistry And Applied Chemistry Kurume National College Of Technology
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YASUDA Takako
Graduate School of Science and Technology, Kobe University
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Yasuda Tatsuji
Institute Of Cellular And Molecular Biology Okayama University Medical School
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Yasuda Takako
Graduate School Of Science And Technology Kobe University
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Inoue Yoko
Department Of Human Sciences Kobe College
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Shiomi Naofumi
Department Of Biosphere Sciences Kobe College
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Shiomi N
Kobe Coll. Hyogo Jpn
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Shiomi N
Department Of Biosphere Sciences Kobe College
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Inoue Yoko
Department of Hematology, Research Institute, International Medical Center of Japan
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Inoue Yoko
Department of Cardiology, Nagoya University Graduate School of Medicine
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