Ascorbic Acid 2-O-α-Glucoside-Induced Redox Modulation in Human Keratinocyte Cell Line, SCC : Mechanisms of Photoprotective Effect against Ultraviolet Light B
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概要
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We previously reported that the topical application of ascorbic acid 2-O-α-glucoside (AA-2G) suppressed the cutaneous inflammation by ultraviolet irradiation in human and guinea pigs (Miyai et al., Nishinihon J. Dermatol., 58,439-443 (1996)). In this paper, the effect of AA-2G on the lethal damage induced by ultraviolet B (UVB) was studied using a human keratinocyte cell line, SCC, established from squamous cell carcinoma. The photoprotective effect of AA-2G on cytotoxicity of UVB in SCC cells was dose dependent (0.125-1 mM) and more effective than that of ascorbic acid (AsA) at 1mM. This protection was completely abolished in the presence of an α-glucosidase inhibitor, castanospermine, indicating that release of AsA from this derivative was essential for reduction of the actinic injury. AA-2G significantly suppressed cytotoxicities of hydrogen peroxide and superoxide anion produced by xanthine and xanthine oxidase. AA-2G exhibited a preventive effect against the cytotoxicity produced by tert-butylhydroperoxide, an inducer of lipid peroxidation, in the presence of α-tocopherol, but not in the absence of α-tocopherol. Cytotoxicity of UVB was also effectively by the combination of AA-2G and α-tocopherol. In addition, AA-2G reduced UVB-promoted formation of lipid peroxide and accumulation of lipofuscin, which is known to be a complex of cellular proteins and metabolites of lipid peroxide.These data suggest that AA-2G prevents the acute inflammation induced by UVB irradiation partly through scavenging reactive oxygen species and protentiating the antioxidative activity of α-tocopherol.
- 社団法人日本薬学会の論文
- 1997-06-15
著者
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YAMAMOTO Itaru
Department of Radiology, Kanazawa Medical University
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Yamamoto Itaru
Okayama Univ. Okayama Jpn
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Yamamoto I
Okayama Univ. Okayama Jpn
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MIYAI Eriko
Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University
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YANAGIDA Mitsuhiro
Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University
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AKIYAMA Jun-ichi
Department of Research and Development, Kaminomoto Co., Ltd.,
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Yamamoto Itaru
Department Of Immunochemistry Faculty Of Pharmaceutical Science Okayama University
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Akiyama J
Department Of Research And Development Kaminomoto Co. Ltd.
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Akiyama Jun-ichi
Department Of Cardiology Kasukabe Shuwa Hospital
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Miyai Eriko
Department Of Immunochemistry Faculty Of Pharmaceutical Sciences Okayama University:department Of Re
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Yanagida M
Department Of Immunochemistry Faculty Of Pharmaceutical Sciences Okayama University:department Of Re
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Yanagida Mitsuhiro
Department Of Gene Mechanisms Grad. Sch. Biostudies Kyoto Univ.
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YANAGIDA MITSUHIRO
Department of Biophysics and Biochemistry, Faculty of Science, Univerisyt of Tokyo
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