pH-Dependent Long-term Radical Scavenging Activity of AA-2G and 6-Octa-AA-2G against 2,2 '-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Radical Cation(Biopharmacy)
スポンサーリンク
概要
- 論文の詳細を見る
The 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS^<・+>) decolorization assay was applied to evaluate the stoichiometric radical scavenging activity of ascorbic acid (AA) and two AA derivatives, 2-O-α-D-glucopyranosyl-L-ascorbic acid (AA-2G) and 2-O-α-D-glucopyranosyl-6-O-octanoyl-L-ascorbic acid (6-Octa-AA-2G). AA rapidly reacted with ABTS^<・+>, and the reaction was completed within 10 min. In contrast, AA-2G and 6-Octa-AA-2G continuously reacted with ABTS^<・+>, and the reaction was not completed after 2 h. The radical scavenging activity of AA-2G and 6-Octa-AA-2G in aqueous solutions at pH 4.0 and above was higher than that at pH 3.0, whereas AA showed no difference in the pH range 3 to 6. The amounts of ABTS^<・+> scavenged by one molecule of AA, AA-2G and 6-Octa-AA-2G after 2 h of reaction at pH 6.0 were approximately 2.0, 3.4 or 3.9 molecules, respectively. This study demonstrates that the quantity of ABTS^<・+> quenched by AA-2G and 6-Octa-AA-2G is superior to that of AA in a long-term reaction.
- 社団法人日本薬学会の論文
- 2003-09-01
著者
-
Yamamoto Itaru
岡山大学 薬学部免疫医薬品化学
-
YAMAMOTO Itaru
Department of Radiology, Kanazawa Medical University
-
Yamamoto I
Okayama Univ. Okayama Jpn
-
TAI Akihiro
Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University
-
TAKEBAYASHI Jun
Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University
-
Yamamoto Itaru
Department Of Immunochemistry Faculty Of Pharmaceutical Science Okayama University
-
Tai A
Department Of Immunochemistry Faculty Of Pharmaceutical Sciences Okayama University
-
Tai Akihiro
Department Of Immunochemistry Division Of Pharmaceutical Sciences Okayama University Graduate School
-
Takebayashi Jun
国立健康･栄養研究所 食品機能プロジェクト
-
Takebayashi Jun
Food Function And Labeling Program Incorporated Administrative Agency National Institute Of Health A
-
Yamamoto I
Department Of Immunochemistry Division Of Pharmaceutical Sciences Okayama University Graduate School
-
Takebayashi Jun
Department Of Immunochemistry Division Of Pharmaceutical Sciences Okayama University Graduate School Of Medicine Dentistry And Pharmaceutical Sciences
-
Tai Akihiko
Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University
関連論文
- Value of whole-body FDG PET in management of lung cancer
- Changes in regional cerebral blood flow in irradiated regions and normal brain after stereotactic radiosurgery
- Single photon emission CT images in a case of intraventricular neurocytoma
- Antiscorbutic Activity of L-Ascorbic Acid 2-Glucoside and Its Availability as a Vitamin C Supplement in Normal Rats and Guinea Pigs
- Formation of a Stable Ascorbic Acid 2-Glucoside by Specific Transglucosylation with Rice Seed α-Glucosidase(Biological Chemistry)
- ACTIVATION OF MURINE LYMPHOCYTES BY 2-MERCAPTOETHANOL AND RELATED THIOL COMPOUNDS AND ITS MECHANISM. II. EFFECT OF THIOL COMPOUNDS ON THE VIABILITY OF THE LYMPHOCYTES UNDER A CYSTINE-DEFICIENT CULTURE CONDITION
- IN VITRO GENERATION OF ANTIGEN-SPECIFIC SUPPRESSOR T CELL ACTIVITY. CULTURE CONDITIONS FOR ABROGATING THE INDUCTION OF NONSPECIFIC SUPPRESSOR T CELLS
- Behavior of 2-O-α-D-Glucopyranosyl-L-ascorbic Acid in a Flour-water Suspension and Its Effectiveness as a Dough Improver
- CT and MRI Findings of Xanthoma in the Orbitofrontal Region
- An isocratic HPLC method for the simultaneous determination of novel stable lipophilic ascorbic acid derivatives and their metabolites
- Characterization of the Radical-Scavenging Reaction of 2-O-Substituted Ascorbic Acid Derivatives, AA-2G, AA-2P, and AA-2S : A Kinetic and Stoichiometric Study(Biopharmacy)
- Inhibition of Hepatocyte Growth Factor Induction in Human Dermal Fibroblasts by Tryptanthrin(Pharmacology)
- pH-Dependent Long-term Radical Scavenging Activity of AA-2G and 6-Octa-AA-2G against 2,2 '-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Radical Cation(Biopharmacy)
- Long-Term Radical Scavenging Activity of AA-2G and 6-Acyl-AA-2G against 1,1-Diphenyl-2-picrylhydrazyl
- Ascorbic Acid 2-O-α-Glucoside-Induced Redox Modulation in Human Keratinocyte Cell Line, SCC : Mechanisms of Photoprotective Effect against Ultraviolet Light B
- Ascorbic Acid 2-O-α-Glucoside, a Stable Form of Ascorbic Acid, Rescues Human Keratinocyte Cell Line, SCC, from Cytotoxicity of Ultraviolet Light B
- Purification and Characterization of a Mouse Hepatocyte Growth-Stimulating Factor from the Liver of Carbon Tetrachloride-Treated Mice
- Inhibition of Hepatocyte Growth Factor Production in Human Fibroblasts by Ursodeoxycholic Acid(Pharmacology)
- Biological and Immunological Properties of Human Hepatocyte Growth Factor from Plasma of Patients with Fulminant Hepatic Failure.
- Vitamin C Activity in Guinea Pigs of 6-O-Acyl-2-O-α-D-glucopyranosyI-L-ascorbic Acids with a Branched-acyl Chain(Food & Nutrition Science)
- Synthesis and Characterization of 6-O-Acyl-2-O-α-D-glucopyranosyl-L-ascorbic Acids with a Branched-acyl Chain
- Bioavailability of a Series of Novel Acylated Ascorbic Acid Derivatives, 6-O-Acyl-2-O-α-D-glucopyranosyl-L-ascorbic Acids, as an Ascorbic Acid Supplement in Rats and Guinea Pigs(Food & Nutrition Sience)
- Radical Scavenging Activity against 1, 1-Diphenyl-2-picrylhydrazyl of Ascorbic Acid 2-Glucoside(AA-2G)and 6-Acyl-AA-2G
- THE USE OF β-CYCLODEXTRIN IN LYMPHOCYTE CULTURE. INDUCTION OF THE PRIMARY ANTIBODY RESPONSE IN VITRO IN CALF SERUM- OR NEWBORN BOVINE SERUM-CONTAINING CULTURE MEDIUM
- ENZYME IMMUNOASSAY OF CYCLIC NUCLEOTIDE
- Activation of Murine Peritoneal Macrophages by Water-Soluble Extracts of Bursaphelenchus xylophilus, a Pine Wood Nematode
- Augmentation by Bursaphelenchus xylophilus, a Pine Wood Nematode, of Polyclonal IgE Production Induced by Lipopolysaccharide Plus Interleukin-4 in Murine Splenocytes
- Gallium-67-citrate scintigraphy of primary renal lymphoma
- Enhancing Effect of 2-O-α-D-Glucopyranosyl-L-ascorbic Acid, a Stable Ascorbic Acid Derivative, on Collagen Synthesis
- Augmentation of Antigen-Specific Antibody Production and IL-10 Generation with a Fraction from Rooibos (Aspalathus linearis) Tea
- DEGRADATION OF HISTAMINE IN THE PRESENCE OF ASCORBIC ACID AND Cu^ ION : INVOLVEMENT OF HYDROGEN PEROXIDE
- Requirement of Cytokines for Augmentation of the Antigen-Specific Antibody Responses by Ascorbate in Cultured Murine T-Cell-Depleted Splenocytes
- Enhancement of Neurite Outgrowth in PC12 Cells Stimulated with Cyclic AMP and NGF by 6-Acylated Ascorbic Acid 2-O-α-Glucosides(6-Acyl-AA-2G),Novel Lipophilic Ascorbate Derivatives
- Synthesis of 2-O-α-D-Glucopyranosyl L-Ascorbic Acid by Cyclomaltodextrin Glucanotransferase from Bacillus stearothermophilus(Biological Chemistry)
- Effects of Rooibos Tea Extract on Antigen-specific Antibody Production and Cytokine Generation in Vitro and in Vivo(Biochemistry & Molecular Biology)
- DEVELOPMENT OF ENZYME IMMUNOASSAY FOR MABUTEROL AND ITS APPLICATION FOR METABOLIC STUDY
- An Increase in Histone Acetylation and IL-2 Antagonizing the Immunoinhibitory Effect Are Necessary for Augmentation by Butyrate of in Vitro Anti-TNP Antibody Production
- In Vitro Augmentation of Natural Killer Activity and Interferon-γ Production in Murine Spleen Cells with Agaricus blazei Fruiting Body Fractions
- Protease-Catalyzed Monoacylation of 2-O-α-D-Glucopyranosyl-L-ascorbic Acid in Pyridine
- ACTIVATION OF ALLOPURINOL OXIDIZING ENZYME ACTIVITY OF THE DIALYSATE OF MOUSE LIVER SUPERNATANT BY MONOVALENT CATIONS
- 2-O-α-D-Glucopyranosyl-L-ascorbic Acid Scavenges 1,1-Diphenyl-2-picrylhydrazyl Radicals via a Covalent Adduct Formation
- Determination of ascorbic acid and its related compounds in foods and beverages by hydrophilic interaction liquid chromatography
- EFFECT OF N-(2-CARBOXYPHENYL)-4-CHLOROANTHRANILIC ACID DISODIUM SALT (CCA) ON THE INDUCTION OF HELPER AND SUPPRESSOR T CELLS IN VITRO AND IN VIVO
- ANTIGEN-INDUCED ELEVATION OF CYCLIC GMP LEVEL IN PLASMA AND ITS ABOLITION IN EXPERIMENTALLY IMMUNOSUPPRESSIVE MICE
- TISSUE DISTRIBUTION AND CHARACTERISTICS OF XANTHINE OXIDASE AND ALLOPURINOL OXIDIZING ENZYME
- 2-O-α-D-Glucopyranosyl-L-ascorbic Acid Scavenges 1,1-Diphenyl-2-picrylhydrazyl Radicals via a Covalent Adduct Formation
- Estimated Average Daily Intake of Antioxidants from Typical Vegetables Consumed in Japan : A Preliminary Study
- Histone Hyperacetylation Plays a Role in Augmentation of IL-4-Induced IgE Production in LPS-Stimulated Murine B-Lymphocytes by Sodium Butyrate
- Augmentation of Antigen-Specific Antibody Production and IL-10 Generation with a Fraction from Rooibos (Aspalathus linearis) Tea
- In Vitro and In Vivo Prolonged Biological Activities of Novel Vitamin C Derivative, 2-O-α-D-Glucopyranosyl-L-Ascorbic Acid (AA-2G), in Cosmetic Fields
- Augmentation of the antibody response by lipoic acid in mice. I. Analysis of the mode of action in an in vitro culture system.
- Abnormality of the delayed type hypersensitivity (DTH) response to Bordetella pertussis in spontaneously hypertensive rats (SHR).
- An experimental model of nephritis induced by calf serum injection in mice.
- In Situ Intestinal Absorption of 2-0-.ALPHA.-D-Glucopyranosyl-L-Ascorbic Acid in Guinea Pigs.
- Vitamin C Activity of 2-O-.ALPHA.-D-Glucopyranosyl-L-Ascorbic Acid in Guinea Pigs.
- Alendronate Modulates Osteogenesis of Human Osteoblastic Cells In Vitro.
- Induction of prolonged elevation of interleukin 2 in the culture medium of murine splenocytes by their precultivation before mitogenic stimulations and its potentiation by glucocorticoid treatment and restraint stress loading.
- Immunomodulatory activity of the newly synthesized compound TOK-8801 (N-(2-phenylethyl)-3,6,6-trimethyl-5,6-dihydroimidazo(2,1-b)thiazole-2-carboxamide).
- The use of .BETA.-cyclodextrin-containing culture medium for in vitro evaluation of immunomodulating agents.
- Augmentation of in vitro antibody response by disulfide compounds. I. Comparison between intermolecular and intramolecular disulfides.
- Butyrate Enhances the In Vitro Anti-SRBC (Sheep Red Blood Cell) Antibody Responses in Murine Splenocytes.
- Inhibition of proliferative responses and interleukin 2 productions by salazosulfapyridine and its metabolites.
- Enhancement by Ascorbic Acid 2-Glucoside or Repeated Additions of Ascorbate of Mitogen-Induced IgM and IgG Productions by Human Peripheral Blood Lymphocytes.
- Augmentation of the antibody response by lipoic acid in mice. II Restoration of the antibody response in immunosuppressed mice.