Crystallization and Structural Analysis of Intact Maltotetraose-forming Exo-amylase from Pseudomonas stutzeri
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概要
- 論文の詳細を見る
The intact maltotetraose-forming exo-amylase from Pseudomonas stutzeri (G4-1), which has a raw starch binding domain, has been crystallized. The structure was identified (PDB entry 1GCY) by the molecular replacement method using the structure of its catalytic domain (G4-2). The result showed that the raw starch binding domain is in a disordered state, the corresponding electron densities being almost invisible. Superposition of these two enzyme forms showed evidence for the possible location of the raw starch binding domain (SBD). This crystal is a novel case, in that it forms a regular lattice incorporating flexibly bound SBD in the channel of crystal packing of the catalytic domains.
- 社団法人日本農芸化学会の論文
- 2001-01-23
著者
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MEZAKI Yoshihiro
Hyogo Prefecture Institute of Industrial Research
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Matsuura Yoshiki
Institute For Protein Research Osaka University
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Kubota Michio
Hayashibara Biochemical Laboratories Inc.
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Katsuya Yoshio
Hyogo Prefectural Institute Of Industrial Research
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Matsuura Y
Institute For Protein Research Osaka University
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Mezaki Yoshihiro
Hyogo Prefectural Institute Of Industrial Research
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