Overproduction and preliminary X-ray characterization of aspartate aminotransferase from Escherichia coli.

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概要

• 論文の詳細を見る

• The aspartate aminotransferase of Escherichia coli was overproduced in cells after genetic manipulation, and was crystallized from a polyethylene glycol solution, pH 7.0. The crystals obtained were of good quality and had diffractions extending beyond 2.4 Å. The space group and unit cell dimensions were determined with a precession camera and a four-circle diffractometer to be C2221, and a=157.1 Å, b=85.5 Å, and c=79.7 Å, respectively. Only one protein subunit is contained in an asymmetric unit.

• 社団法人 日本生化学会の論文

著者

• Kusunoki Masami

  Institute For Protein Research Osaka University

• Matsuura Yoshiki

  Institute For Protein Research Osaka University

• Kagamiyama Hiroyuki

  Department Of Biochemistry Osaka Medical College

• Kuramitsu Seiki

  Department Of Bioligy Graduate School Of Science Osaka University

• KAMITORI Shigehiro

  Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology

• Hirotsu Ken

  Department Of Chemistry Faculty Of Science Osaka City University

• Kondo Kiyoshi

  Department Of Immunochemistry Research Institute For Microbial Diseases Osaka University

• Higuchi Taiichi

  Department Of Chemistry Faculty Of Science Osaka City University

• Yasuoka Noritake

  Basic Research Laboratory, Himeji Institute of Technology

• Higuchi Yoshiki

  Basic Research Laboratory, Himeji Institute of Technology

• INOUE Katsura

  Department of Medical Chemistry,Osaka Medical College

• Inoue Katsura

  Department of Medical Chemistry, Osaka Medical College

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