Identification and characterization of Thermus thermophilus HB8 RuvA protein, the subunit of the RuvAB protein complex that promotes branch migration of Holliday junctions

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概要

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• The Escherichia coli ruvA and ruvB genes constitute an SOS-regulated operon. The products of these genes form a protein complex that promotes branch migration of the Holliday junction, an intermediate of homologous recombination. RuvA protein binds specifically to the Holliday junction and recruits RuvB protein to the junction. RuvB is an ATP-driven motor protein involved in branch migration. We previously cloned the ruvB gene of the thermophilic bacterium Thermus thermophilus HB8 (Tth) and found that, in contrast to the operon structure in most mesothermic bacteria, the ruvA gene is absent from the vicinity of ruvB. In this work, we cloned the ruvA gene from T. thermophilus HB8 and analyzed its nucleotide sequence. Tth RuvA is a protein of 20,414 Da consisting of 191 amino acid residues, and is 37% identical in amino acid sequence to E. coli RuvA. Tth ruvA complemented the DNA repair defect of E. coli ΔruvA mutants. The purified Tth RuvA protein stimulated Tth RuvB activities, such as hydrolysis of ATP and promotion of branch migration of the Holliday junction, in a manner similar to the RuvA-RuvB interactions observed in E. coli. In addition, Tth RuvA stimulated the E. coli RuvB activities in vitro, which was well consistent with the results of in vivo hetero-complementation experiments.

• 日本遺伝学会の論文

著者

• KURAMITSU Seiki

  Department of Biology,Graduate School of Science,Osaka University

• Shinagawa Hideo

  Maxillofacial Orthognathics, Graduate School, Tokyo Medical and Dental University

• ISHINO Yoshizumi

  Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University

• Ohnishi Takayuki

  Department of Caldiology, Hiratsuka Kyousai Heart Center, Hiratsuka Kyosai Hospital

• Shinagawa Hideo

  Department of Biomedical Sciences, University of Maryland Baltimore

• Kuramitsu Seiki

  Department Of Bioligy Graduate School Of Science Osaka University

• NAKATA Atsuo

  Department of Experimental Chemotherapy, The Research Institute for Microbial Disease, Osaka Univers

• Nakata Atsuo

  Department Of Marine Biotechnology Faculty Of Technology Fukuyama University

• IWASAKI Hiroshi

  PRESTO, JST

• Ohnishi Takayuki

  Department Of Molecular Microbiology Research Institute For Microbial Diseases Osaka University

• Shinagawa H

  Maxillofacial Orthognathics Graduate School Tokyo Medical And Dental University

• Shinagawa Hideo

  Department Of Biomedical Sciences University Of Maryland Baltimore

• Ishino Y

  Department Of Genetic Resources Technology Faculty Of Agriculture Kyushu University

• Ishino Yoshizumi

  Department Of Genetic Resources Technology Graduate School Of Bioresource And Bioenvironmental Scien

• Ishino Yoshizumi

  Department Of Bioscience And Biotechnology Graduate School Of Bioresource And Bioenvironmental Scien

• Iwasaki Hiroshi

  Graduate School Of Integrated Science Yokohama City University

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