Novel endonuclease in Archaea cleaving DNA with various branched structure

スポンサーリンク

概要

• 論文の詳細を見る

• We identified a novel structure-specific endonuclease in Pyrococcus furiosus. This nuclease contains two distinct domains, which are similar to the DEAH helicase family at the N-terminal two-third and the XPF endonuclease superfamily at the C-terminal one-third of the protein, respectively. The C-terminal domain has an endonuclease activity cleaving the DNA strand at the 5-side of nicked or flapped positions in the duplex DNA. The nuclease also incises in the proximity of the 5-side of a branch point in the template strand for leading synthesis in the fork-structured DNA. The N-terminal helicase may work cooperatively to change the fork structure suitable for cleavage by the C-terminal endonuclease. This protein, designated as Hef (helicase-associated endonuclease for fork-structured DNA), may be a prototypical enzyme for resolving stalled forks during DNA replication, as well as working at nucleotide excision repair.

• 日本遺伝学会の論文
• 2002-08-25

著者

• ISHINO Yoshizumi

  Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University

• Shinagawa Hideo

  Department Of Biomedical Sciences University Of Maryland Baltimore

• Komori Kayoko

  Department Of Molecular Biology Biomolecular Engineering Research Institute

• FUJIKANE Ryosuke

  Department of Molecular Biology, Biomolecular Engineering Research Institute

• Ishino Yoshizumi

  Department Of Genetic Resources Technology Graduate School Of Bioresource And Bioenvironmental Scien

• Ishino Yoshizumi

  Department Of Molecular Biology Biomolecular Engineering Research Institute

• Fujikane Ryosuke

  Department Of Molecular Biology Biomolecular Engineering Research Institute

• Ishino Yoshizumi

  Department Of Bioscience And Biotechnology Graduate School Of Bioresource And Bioenvironmental Scien

関連論文

• The 4^ International Symposium on 3R ; DNA Replicaiton, Recombination and Repair
• Tongue muscle fiber tracking during rest and tongue protrusion with oral appliances : A preliminary study with diffusion tensor imaging
• Genetic analysis of DNA repair in the hyperthermophilic archaeon, Thermococcus kodakaraensis
• Identification and characterization of Thermus thermophilus HB8 RuvA protein, the subunit of the RuvAB protein complex that promotes branch migration of Holliday junctions
• Complete nucleotide sequence of the prophage VT2-Sakai carrying the verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157 : H7 derived from the Sakai outbreak
• Three Antigenic Regions in p17 of Human Immunodeficiency Virus Type 1 ( HIV-1 ) Revealed by Mouse Monoclonal Antibodies and Human Antibodies in HIV-1 Carriar Sera
• Identification of the critical region in Replication factor C from Pyrococcus furiosus for the stable complex formation with Proliferating cell nuclear antigen and DNA
• Overproduction, Purification, and Diagnostic Use of the Recombinant HIV-1 Gag Proteins, the Precursor Protein p55 and the Processed Products p17, p24, and p15
• A useful strategy to construct DNA polymerases with different properties by using genetic resources from environmental DNA
• SIMULATION OF A TRANSIENT HETEROGENEOUS PHASE TRANSITION
• DETERMINING THE PHASE TRANSITION OF CONDENSATE FORMED BY HETEROGENEOUS NUCLEATION OF CONDENSABLE VAPORS ONTO A COLD SUBSTRATE
• HETEROGENEOUS NUCLEATION CHARACTERISTICS OF MIXED VAPOR OF BENZENE AND CYCLOHEXANE ON A COLD SUBSTRATE
• OBSERVATIONS OF HETEROGENEOUS NUCLEATION OF WATER VAPOR ON A COLD SUBSTRATE
• DETERMINATION OF THE CRITICAL SUPERSATURATION RATIO IN HETEROGENEOUS NUCLEATION OF BENZENE VAPOR ON A COLD SUBSTRATE
• Roles of the recG gene product of Escherichia coli in recombination repair : Effects of the ΔrecG mutation on cell division and chromosome partition
• Novel endonuclease in Archaea cleaving DNA with various branched structure
• Molecular Flow Analysis in Orifice-Restricted Tube Using Maxwellian Distribution Function
• Analysis of a Stationaxy-Phase Specific Promoter Recognized by Eσ^
• The euryarchaeotes, a subdomain of Archaea, survive on a single DNA polymerase : Fact or farce?
• A Free Molecular Flow Characteristic through a Conduit with an Orifice-Restricted Exit
• The euryarchaeotes, a subdomain of Archaea, survive on a single DNA polymerase:Fact or Farce?
• Genetic analysis of DNA repair in the hyperthermophilic archaeon, Thermococcus kodakaraensis
• 3H1024 P24 Analysis on dynamical structure of intrinsically disordered protein Hef, using MD-SAXS method(3H Protein: Property 4,The 49th Annual Meeting of the Biophysical Society of Japan)
• Molecular Flow Analysis in Orifice-Containing Tube Using Maxwellian Distribution Function.
• The analysis of flow of rarefied gases through packed beds by monte carlo method.
• Analysis of pressure distribution for free molecule flow in circular tube.
• Microstructure and permeability of dried product for frozen aqueous solution.
• OBSERVATIONS OF TOPOLOGICAL CHARACTERISTICS OF CAMPHOR CRYSTAL
• Purification and characterization of the Escherichia coli oxyR protein, the positive regulator for a hydrogen peroxide-inducible regulon.
• The OsGEN-L protein from Oryza sativa possesses Holliday junction resolvase activity as well as 5'-flap endonuclease activity

もっと見る 閉じる

スポンサーリンク