Identification of the critical region in Replication factor C from Pyrococcus furiosus for the stable complex formation with Proliferating cell nuclear antigen and DNA
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概要
- 論文の詳細を見る
Replication factor C (RFC) and proliferating cell nuclear antigen (PCNA) are accessory proteins essential for processive DNA synthesis. The function of RFC is to load PCNA, a processivity factor of replicative DNA polymerases, onto primed DNA templates. The central hole of the PCNA homo-trimeric ring encircles doublestranded DNA, so that DNA polymerases can operate for DNA synthesis with PCNA along a DNA template. The Pyrococcus furiosus RFC (PfuRFC) consists of a small subunit (RFCS, 37kDa) and a large subunit (RFCL, 55kDa), which show significant sequence identity to the eukaryotic homologs. The C-terminal region of RFCL has an acidic cluster of about 30 amino acids, which consists mainly of glutamic acid residues, and a following basic cluster of 10 amino acids, which consists mainly of lysine residues. These clusters of charged amino acids, which precede the C-terminal consensus sequence, PIP (PCNA interacting protein)-box, are conserved in several archaeal RFCLs. The series of mutant PfuRFC containing the C-terminal deletions in RFCL were constructed. The mutational analyses showed that the charged cluster is not essential for loading of PCNA onto DNA. However, the region containing the basic cluster is important for the stable ternary (RFC-PCNA-DNA) complex formation.
- 日本遺伝学会の論文
- 2005-04-25
著者
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ISHINO Yoshizumi
Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University
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Miyata Tomoko
Grad. Sch. Of Front. Biosci. Osaka Univ.
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Miyata Tomoko
Department Of Pathology And Clinical Laboratories Nagoya University Hospital
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Morikawa Kosuke
Osaka Univ.:crest Jst
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Morikawa Kosuke
Ipr Osaka Univ.
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Morikawa Kosuke
Department of Structural Biology, Biomolecular Engineering Research Institute (BERI)
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Nishida Hirokazu
Hitachi, Ltd.
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NISHIDA Hirokazu
Department of Structural Biology, Biomolecular Engineering Research Institute (BERI)
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ISHINO Sonoko
Department of Molecular Biology, Biomolecular Engineering Research Institute (BERI)
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Ishino Sonoko
Department Of Molecular Biology Biomolecular Engineering Research Institute (beri)
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Nishida Hirokazu
Hitachi Ltd.
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Nishida Hirokazu
Central Research Lab. Hitachi Ltd.
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Ishino Yoshizumi
Kyusyu University
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Ishino Yoshizumi
Department Of Genetic Resources Technology Graduate School Of Bioresource And Bioenvironmental Scien
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Ishino Yoshizumi
Fac. Of Agriculture Kyushu Univ.
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Ishino Yoshizumi
Department Of Bioscience And Biotechnology Graduate School Of Bioresource And Bioenvironmental Scien
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Ishino Sonoko
Department Of Bioscience And Biotechnology Graduate School Of Bioresource And Bioenvironmental Scien
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NISHIDA HIROKAZU
Department of Chemical Engineering, University of Osaka Prefecture
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