Purification and Properties of Carnitine Dehydrogenase form Pseudomonas sp. YS-240
スポンサーリンク
概要
- 論文の詳細を見る
Carnitine dehydorogenase from Pseudomonas sp. YS-240 was purified to apparent homogeneity. Purification was achieved by ion-exchange chromatography, followed by ammonium sulfate fractionation, hydroxylapatite chromatography and gel filtration. This enzyme had the molecular weight of 110 kDa and consisted of two identical subunits. The isoelectric point was found to be 5.5. The optimum pH for enzymatic activity in the oxidation reaction was found to be 9.5. The enzyme was hightly specific for L-carnitine and NAD^+. The Michaelis constants for L-carnitine and NAD^+ were 1.2 mM and 0.1 mM, respectively.
- 社団法人日本生物工学会の論文
- 1994-11-25
著者
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Mitsuzumi Hitoshi
Department of Agricultural, Biological, and Environmental Sciences, Faculty of Agriculture, Tottori
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MORI Nobuhiro
Department of Agricultural, Biological, and Environmental Sciences, Faculty of Agriculture, Tottori
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Mori N
Hitachi Plant Engineering & Construction Corp. Chiba Jpn
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Mori N
Tottori Univ. Tottori Jpn
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KITAMOTO Yutaka
Department of Bioscience and Biotechnology, Faculty of Agriculture, Tottori University
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Kitamoto Y
Tottori Univ. Tottori Jpn
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Kitamoto Yutaka
Department Of Agricucultural Chemistry Faculty Of Agriculture Tottori University
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Kitamoto Yutaka
Science Laboratory Asano Industry Co. Ltd.
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Kitamoto Yutaka
Laboratory Of Microbial Biotechnology Department Of Biochemistry And Biotechnology Faculty Of Agricu
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MITSUZUMI Hitoshi
Hayashibara Biochemical Laboratories, Inc.
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Mitsuzumi Hitoshi
Hayashibara Biochemical Laboratories Inc.
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Mitsuzumi Hitoshi
Department Of Agricultural Chemistry Faculty Of Agriculture Tottori Universtiy
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Kitamoto Yutaka
Department Of Bioscience And Biotechnology Faculty Of Agriculture Tottori University
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Mori N
The United Graduate School Of Agricultural Sciences Tottori University
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Mori Nobuhiro
Department Of Agricultural Biological And Environmental Sciences Faculty Of Agriculture Tottori Univ
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Mitsuzumi Hitoshi
Department Of Agricultural Biological And Environmental Sciences Faculty Of Agriculture Tottori Univ
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