Purification and Properties of Propanediol Dehydratase from Propionibacterium freudenreichii
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概要
- 論文の詳細を見る
Propanediol dehydratase was purified from cell extracts of Propionibacterium freudenreichii by protamine sulfate treatment, ammonium sulfate fractionation, DEAE-cellulose column chromatography, and Sepharose 4B column chromatography.The enzyme was purified approximately 635-fold with overall recovery of 23% and proved to be almost homogeneous by polyacrylamide gel electrophoresis.The molecular weight of the enzyme was estimated to be 230,000 by gel filtration. The enzyme showed the highest activity around pH 9.2. The enzyme dehydrated DL-1,2-propanediol, 1,2-ethanediol, DL-1,2-butanediol, DL-2,3-butanediol, and DL-1,3-butanediol. The K_m values for 1,2-propanediol, 1,2-ethanediol, and coenzyme B_<12> were 0.18 mM, 1.79 mM, and 0.81 μM, respectively. Propanediol dehydratase was inhibited by sulfhydryl compounds.
- 社団法人日本生物工学会の論文
- 1985-04-25
著者
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KITAMOTO YUTAKA
Faculty of Agriculture, Tottori University
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Mori N
Hitachi Plant Engineering & Construction Corp. Chiba Jpn
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Kitamoto Y
Tottori Univ. Tottori Jpn
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Kitamoto Yutaka
Faculty Of Agriculture Tottori University
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Kitamoto Yutaka
Department Of Agricucultural Chemistry Faculty Of Agriculture Tottori University
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Kitamoto Yutaka
Science Laboratory Asano Industry Co. Ltd.
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Kitamoto Yutaka
Laboratory Of Microbial Biotechnology Department Of Biochemistry And Biotechnology Faculty Of Agricu
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Kitamoto Yutaka
Department Of Bioscience And Biotechnology Faculty Of Agriculture Tottori University
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MORI Nobuhiro
Faculty of Agriculture, Tottori University
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ICHIKAWA Yoshio
Faculty of Agriculture, Tottori University
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HOSOI NOBORU
Faculty of Agriculture, Tottori University, Koyama
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HORIKE YASUYUKI
Faculty of Agriculture, Tottori University
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Hosoi Noboru
Department of Agricultural Chemistry, Faculty of Agriculture, Tottori University
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Mori Nobuhiro
Faculty Of Agriculture Tottori University
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Hosoi Noboru
Department Of Agricultural Chemistry Faculty Of Agriculture Tottori University
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Ichikawa Yoshio
Faculty Of Agriculture Tottori University
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Horike Yasuyuki
Faculty Of Agriculture Tottori University
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