Purificatin and Some Properties of a Carboxymethyl Cellulase from Favolus arcularius
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概要
- 論文の詳細を見る
Favolus arcularius, a wood-rotting basidiomycete, produced carboxymethyl cellulose-hydrolyzing enzymes (CMCases) in culture media. Three main peaks of CMCase activity were separated as CMCase I, II and III at pHs from 4.4 to 5.2 by isoelectric focusing. Further, CMCase IIIa was purified from the CMCase III fraction. The molecular weight of CMCase IIIa was determined to be about 28,000 by SDS-polyacrylamide gel electrophoresis. The enzyme was not active on acivel, cellobiose and laminarin, but could randomly hydrolyze cellooligosaccharides to form G_1 and G_2 units as the end products. The apparent K_m value of the enzyme against CMC was 0.28%.
- 社団法人日本生物工学会の論文
- 1992-03-25
著者
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KITAMOTO YUTAKA
Faculty of Agriculture, Tottori University
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Kitamoto Yutaka
Faculty Of Agriculture Tottori University
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MORI Nobuhiro
Faculty of Agriculture, Tottori University
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ENOKIBARA SHOUGO
Faculty of Agriculture, Tottori University
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Mori Nobuhiro
Faculty Of Agriculture Tottori University
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Enokibara S
Kobe Univ. Commerce Kobe Jpn
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