Absence of the Functional Myosin Heavy Chain 2b Isoform in Equine Skeletal Muscles(Animal Diversity and Evolution)
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概要
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Nucleotide sequences which included the full coding region for three types of myosin heavy chain (MyHC) isoforms were determined from equine skeletal muscles. The deduced amino acid sequences were 1937, 1938, and 1935 residues for the MyHC-2a, -2x, and -slow, respectively. No MyHC-2b isoform was amplified from the equine muscle cDNA except for one pseudogene fragment. One nucleotide was inserted in the coding region of the equine pseudogene product, a minute amount of which was expressed in the skeletal muscle. The 596 bp sequence of the equine MyHC pseudogene was categorized into the MyHC-2b genes on the phylogenetic tree of the mammalian MyHC genes. These results suggest that an ancestral MyHC-2b gene had lost its function and changed to a pseudogene during the course of horse history. The MyHC genes in some ungulates were analyzed through the PCR amplifications using the MyHC isoform-specific primers to confirm the presence of the MyHC-2b and -2x genes. The exon coding the 3' untranslated region of the MyHC-2x was successfully amplified from the all ungulates examined; however, that of the MyHC-2b gene was amplified only from horses, pigs and lesser mouse deer. The PCR analyses from rhinoceros, sika deer, moose, giraffes, water buffalo, bovine, Japanese serow and sheep genes implied the absence of the MyHC-2b-specific sequence in their genomes. These results suggest that the MyHC-2b gene independently lost its function in some ungulate species.
- 社団法人日本動物学会の論文
- 2004-05-25
著者
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Muroya Susumu
National Inst. Of Livestock And Grassland Sci.
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Chikuni K
National Food Res. Inst. Ministry Of Agriculture Forestry And Fisheries Ibaraki Jpn
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Chikuni Koichi
Meat Protein Research Team National Institute Of Livestock And Grassland Science
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Muroya Susumu
Meat Protein Research Team National Institute Of Livestock And Grassland Science
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Nakajima Ikuyo
National Institute Of Livestock And Grassland Science
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Nakajima Ikuyo
Meat Science Laboratory, National Institute of Livestock and Grassland Science
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Nakajima Ikuyo
National Institute of Livestock and Grassland Science, University of Tsukuba
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