卵細胞質内注入法を用いた牛の核移植

スポンサーリンク

概要

• 論文の詳細を見る

• This study investigated bovine somatic nucleus transplantation (NT) using an intracytoplasmic injection system in which cumulus cell nuclei were injected into enucleated oocytes by a piezo micromanipulator. Activation of NT embryos was carried out by (1) simultaneous injection of 2-4 pl of inositol 1,4,5 tri-phosphate (IP3) and a donor nucleus into a recipient cytoplasm (IP method), (2) IP method followed by 1.9 mM 6-dimethylaminopurine (DM) treatment for 3.5 h (IP+DM method), or (3) activated after NT by an ionomycin treatment (IA) followed by DM treatment (IA+DM method). We found the frequency of remodeling nuclei and development into blastocyst stage of NT embryos to be: IP, 44% and 9%; IP+DM, 58% and 29%; and IA+DM, 61% and 27%. These results indicate that IP3 injection into the intracytoplasm followed by DM treatment produces a similar bovine oocyte parthenogenetic activation as IA+DM activation treatment, and that intracytoplasmic nucleus injection is an effective method for bovine somatic NT.

• 日本繁殖生物学会の論文

著者

• 牛島 仁

  千葉県畜産総合研究センター

• 牛島 仁

  Chiba Prefectural Animal Experimental Station

• 石田 和昭

  Chiba Prefectural Livestock Experimental Center

• 長嶋 比呂志

  Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji Un

• NAGASHIMA Hiroshi

  Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji U

• 石田 和昭

  千葉県畜産総合研究センター

• 長嶋 比呂志

  千葉県畜産総合研究センター

• Nagashima Hiroshi

  Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive

• USHIJIMA Hitoshi

  Chiba Prefectural Animal Experimental Station

• ISHIDA Kazuaki

  Chiba Prefectural Livestock Experimental Center

• Nagashima Hiroshi

  Laboratory For Evolutionary Morphology Riken Cdb

関連論文

• ウシ体外成熟卵と凍結精子による卵細胞質内精子注入の現状と展望
• 乳用種未経産牛を用いた受卵牛の効率的な発情排卵同期化
• 発生工学の家畜生産への応用に関する研究
• ブタの子宮角深部注入(DIUI)法による人工授精と従来法の比較
• ブタ生体回収由来ガラス化胚の生存性の改善
• 卵細胞質内注入法を用いた牛の核移植
• 牛8-64細胞期胚割球ならびに胚盤胞内細胞塊細胞核由来再構築胚の体外発生能
• Developmental Ability of Porcine In Vitro Matured Oocytes at the Meiosis II Stage After Vitrification
• 未成熟雄マウス由来 first-wave 円形精子細胞を用いた顕微授精
• 卵細胞質内注入法を用いた牛の核移植
• Production of Cloned Pigs by Nuclear Transfer of Preadipocytes Following Cell Cycle Synchronization by Differentiation Induction
• Cloned Porcine Embryos can Maintain Developmental Ability after Cryopreservation at the Morula Stage
• 体外受精,単為発生および核移植により作出された牛胚(卵)の核の経時的変化
• ショ糖により耐凍剤除去されたウシ凍結胚のIn Vitro生存性
• ガラス針を用いた簡単な顕微操作による牛分離胚の作出
• ガラス針,金属針,およびカミソリ刃により切断2分離した牛分離胚のin vitro生存性
• 牛1卵性2分離胚の用いた胚の性別予知
• Production Efficiency and Telomere Length of the Cloned Pigs Following Serial Somatic Cell Nuclear Transfer
• ヒト血清アルブミンとEGFPを発現する形質転換ブタの開発
• Classification of Morphological Changes Based on the Number of Cleavage Divisions in Bovine Embryos
• 電気的活性化後のブタ体外成熟卵の発生能力
• 性予知胚を用いた牛一卵性双生子の生産
• Calcium Release at Fertilization : Artificially Mimicking the Oocyte's Response to Sperm
• ブタ卵の成熟条件がMII期紡錘体の形態に及ぼす影響
• Transition of Cell Numbers in Bovine Preimplantation Embryos : In Vivo Collected and In Vitro Produced Embryos
• ブタ生体回収由来ガラス化胚の生存性の改善
• Production of Transgenic and Non-Transgenic Clones in Miniature Pigs by Somatic Cell Nuclear Transfer
• Production of Cloned Pigs by Nuclear Transfer of Preadipocytes Following Cell Cycle Synchronization by Differentiation Induction
• Effects of Sperm Pretreatment on Efficiency of ICSI-Mediated Gene Transfer in Pigs
• Association Between Embryonic Loss and Damage to the Zona Pellucida by Invasive Micromanipulation During Oviductal Transfer of Early-Stage Embryos in Pigs
• Effects of Maturation Conditions on Spindle Morphology in Porcine MII Oocytes
• Effects of Recloning on the Efficiency of Production of α1,3-Galactosyltransferase Knockout Pigs
• Production of a Transgenic Pig Expressing Human Albumin and Enhanced Green Fluorescent Protein
• Autopodial Development in the Sea Turtles Chelonia mydas and Caretta caretta
• Application of Genetically Modified and Cloned Pigs in Translational Research
• In Vitro Development and Postvitrification Survival of Cloned Feline Embryos Derived from Preadipocytes
• Development and Perspectives of Perfusion Treatment for Liver Failure
• Cloned Porcine Embryos can Maintain Developmental Ability after Cryopreservation at the Morula Stage
• What Triggered Turtle Shell Evolution? : Identification of Gene Co-Option in Carapace Formation(Organogenesis and Evolution,Symposium,PROCEEDING OF THE 75^ ANNUAL MEETING OF THE ZOOLOGICAL SOCIETY OF JAPAN)
• Bovine Nuclear Transplantation Using a Parthenogenetically Activated Oocyte as the Recipient Cytoplast
• Production of a Calf from a Nuclear Transfer Embryo Using in Vitro Matured Oocytes
• The Production of a Diabetic Mouse Using Constructs Encoding Porcine Insulin Promoter-Driven Mutant Human Hepatocyte Nuclear Factor-1α
• Hollow Fiber Vitrification: A Novel Method for Vitrifying Multiple Embryos in a Single Device
• Hollow Fiber Vitrification : A Novel Method for Vitrifying Multiple Embryos in a Single Device
• Production of Diabetic Offspring Using Cryopreserved Epididymal Sperm by In Vitro Fertilization and Intrafallopian Insemination Techniques in Transgenic Pigs
• In Vitro Development of Porcine Nuclear Transfer Embryos Reconstructed by Intracytoplasmic Microinjection of Cumulus Cell Nuclei into In Vitro Matured Oocytes.
• Evaluation of the Quality of Porcine Somatic Cell Nuclear Transfer Embryo by Gene Transcription Profiles
• The Production of a Diabetic Mouse Using Constructs Encoding Porcine Insulin Promoter-Driven Mutant Human Hepatocyte Nuclear Factor-1.ALPHA.
• Production of Transgenic-cloned Pigs Expressing Far-red Fluorescent Protein, Monomeric Plum

もっと見る 閉じる

スポンサーリンク