Transition of Cell Numbers in Bovine Preimplantation Embryos : In Vivo Collected and In Vitro Produced Embryos

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概要

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• The total cell numbers (TCNs) of bovine embryos collected from superovulated donors (VIVO embryos) were counted 0-9 d after ovulation to quantify the developmental process. Using numerical analysis of embryo development, we also compared the developmental process of VIVO embryos, in vitro-fertilized (IVF) embryos and nuclear transfer (NT) embryos obtained from enucleated oocytes and blastomere nuclei. The TCNs of embryos were measured using the air-dry method. Cleavage divisions (CD) of the embryos were obtained using logarithmic transformation of the TCN. The TCN of the VIVO embryos increased significantly (P<0.001) with time. The relationship between the CD of the VIVO embryos at 0-9 d after ovulation and age in days was described by a linear equation with a high correlation (y=1.03 x+0.16, r=0.99), showing that CD occurs about once each day for all blastomeres. However, compared to the VIVO embryos, the TCN of the IVF embryos did not increase from 3-4 d nor after 7 d; the TCN of the NT embryos did not increase after 7 d (P>0.05). The results suggest a delay in development at these developmental stages. The slopes of regression lines of the IVF and NT embryos were significantly (P<0.001) smaller, indicating that quantification of the developmental process of VIVO embryos according to TCN and CD would be useful as criteria for numerical evaluation of the developmental process of bovine in vitro produced embryos.

• 日本繁殖生物学会の論文
• 2008-08-01

著者

• AKIYAMA Kiyoshi

  Kanagawa Prefectural Livestock Industry Technology Center

• USHIJIMA Hitoshi

  Chiba Prefectural Animal Experimental Station

• TAJIMA Toshio

  Chiba Prefectural Livestock Experimental Station

• Akiyama Kiyoshi

  Kanagawa Prefectural Livestock Industry Technical Center

• Ushijima Hitoshi

  Chiba Prefectural Ichihara Dairy Experimental Station

関連論文

• ブタ生体回収由来ガラス化胚の生存性の改善
• 卵細胞質内注入法を用いた牛の核移植
• Viability of Porcine Embryos after Vitrification Using Water-soluble Pullulan Films
• Viability of Porcine Embryos after Vitrification Using Water-soluble Pullulan Films
• Classification of Morphological Changes Based on the Number of Cleavage Divisions in Bovine Embryos
• Transition of Cell Numbers in Bovine Preimplantation Embryos : In Vivo Collected and In Vitro Produced Embryos
• ブタ生体回収由来ガラス化胚の生存性の改善
• Calf production from vitrified bovine sexed embryos following in-straw dilution
• Bovine Nuclear Transplantation Using a Parthenogenetically Activated Oocyte as the Recipient Cytoplast
• Production of a Calf from a Nuclear Transfer Embryo Using in Vitro Matured Oocytes
• Effect of cellooligosaccharide or synbiotic feeding on growth performance, fecal condition and hormone concentrations in Holstein calves
• Effects of growth and dietary crude protein level until first insemination on milk production during first lactation in Holstein heifers

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