Production of a Calf from a Nuclear Transfer Embryo Using <I>in Vitro</I> Matured Oocytes

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概要

• 論文の詳細を見る

• This study was conducted to examine the possibility of nuclear transplantation using the bovine oocytes matured <I>in vitro</I>. Sixteen-cell stage embryos (fresh, frozen or embryos developed from the oocytes matured and fertilized <I>in vitro</I>) were designated to donor cells. The follicular oocytes matured <I>in vitro</I> were used as enucleated recipient oocytes. The enucleated oocytes were fused with a blastomere from donor embryos by electrofusion. Most recipient oocytes (325/417, 78%) fused with donor cells. The reconstituted eggs developed to 2-cell stage when cultured on a layer of cumulus cells (187, 58%). Out of the 325 reconstituted eggs, 35(11%) developed into morulae and 9 (3%) into blastocysts stage respectively. Eighteen morula or blastocyst stage embryos were transferred nonsurgically to 9 recipient cows into 1-3 embryos per recipient. Two recipients were confirmed pregnant. One of recipients produced a live offspring resulting from a fresh donor blastomere. The present study showed that <I>in vitro</I> matured oocytes can be used as recipient cytoplasm.

• 日本繁殖生物学会の論文

著者

• USHIJIMA Hitoshi

  Chiba Prefectural Animal Experimental Station

• ETO T.

  Chiba Prefectural Livestock Experiment Station Yachimata Takidai

関連論文

• ブタ生体回収由来ガラス化胚の生存性の改善
• 卵細胞質内注入法を用いた牛の核移植
• Classification of Morphological Changes Based on the Number of Cleavage Divisions in Bovine Embryos
• Transition of Cell Numbers in Bovine Preimplantation Embryos : In Vivo Collected and In Vitro Produced Embryos
• ブタ生体回収由来ガラス化胚の生存性の改善
• Bovine Nuclear Transplantation Using a Parthenogenetically Activated Oocyte as the Recipient Cytoplast
• Production of a Calf from a Nuclear Transfer Embryo Using in Vitro Matured Oocytes

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