Production Efficiency and Telomere Length of the Cloned Pigs Following Serial Somatic Cell Nuclear Transfer
スポンサーリンク
概要
- 論文の詳細を見る
The aim of the present study was to examine the production efficiency of cloned pigs by serial somatic cell nuclear transfer (SCNT) and to ascertain any changes in the telomere lengths of multiple generations of pigs. Using fetal fibroblasts as the starting nuclear donor cells, porcine salivary gland progenitor cells were collected from the resultant first-generation cloned pigs to successively produce second- and third-generation clones, with no significant differences in production efficiency, which ranged from 1.4% (2/140) to 3.3% (13/391) among the 3 generations. The average telomere lengths (terminal restriction fragment values) for the first, second and third generation clones were 16.3, 18.1 and 20.5 kb, respectively, and were comparable to those in age-matched controls. These findings suggest that third-generation cloned pigs can be produced by serial somatic cell cloning without compromising production efficiency and that the telomere lengths of cloned pigs from the first to third generations are normal.
- 日本繁殖生物学会の論文
- 2008-08-01
著者
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UENO Satoshi
Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji U
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NAGASHIMA Hiroshi
Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji U
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Okumura Kenji
Department of Cardiology, Nagoya University School of Medicine
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TOMII Ryo
Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji Un
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KUROME Mayuko
Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji Un
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Ueno Satoshi
Lab. Of Developmental Engineering Dep. Of Life Sciences School Of Agriculture Meiji Univ.
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ENDO Fumio
Department of Pediatrics, Kumamoto University Medical School
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MATSUMOTO Mitsuhito
National Agricultural Research Center for Kyushu Okinawa Region
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KAJI Yuji
National Agricultural Research Center for Kyushu Okinawa Region
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Okumura Kenji
Department O Pediatrics Graduate School Of Medical Science Kumamoto University
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NAKAMURA Kimitoshi
Department of Pediatrics, Kumamoto University School of Medicine
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HISATOMI Hisashi
Laboratory of Cellular and Molecular Biochemistry, Department of Materials and Life Science, Seikei
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MATSUMOTO Shirou
Department o Pediatrics, Graduate School of Medical Science, Kumamoto University
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HIRUMA Katsumi
Laboratory of Developmental Engineering Department of Life Science, School of Agriculture, Meiji Uni
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SAITO Hitoshi
Laboratory of Developmental Engineering Department of Life Science, School of Agriculture, Meiji Uni
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Endo Fumio
Department O Pediatrics Graduate School Of Medical Science Kumamoto University
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Nagashima Hiroshi
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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Kaji Yuji
National Agricultural Res. Center For Kyushu Okinawa Region
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Tomii Ryo
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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Ueno Satoshi
Laboratory Of Developmental Engineering Department Of Life Science Meiji University
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Hisatomi Hisashi
Laboratory Of Cellular And Molecular Biochemistry Department Of Materials And Life Science Seikei Un
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Kurome Mayuko
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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Hiruma Katsumi
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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Saitoh Hitoshi
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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Nakamura Kimitoshi
Department O Pediatrics Graduate School Of Medical Science Kumamoto University
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Nagashima Hiroshi
Lab. Of Developmental Engineering Dep. Of Life Sciences School Of Agriculture Meiji Univ.
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Matsumoto Shirou
Department O Pediatrics Graduate School Of Medical Science Kumamoto University
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Nagashima Hiroshi
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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Kurome Mayuko
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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Nagashima Hiroshi
Laboratory For Evolutionary Morphology Riken Cdb
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