Association Between Embryonic Loss and Damage to the Zona Pellucida by Invasive Micromanipulation During Oviductal Transfer of Early-Stage Embryos in Pigs
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概要
- 論文の詳細を見る
The aim of this study was to examine the impact of zona pellucida damage, which might arise during somatic cell nuclear transfer (SCNT), on the development and survival of transferred embryos. The zonae pellucidae of in vitro matured oocytes were either punctured with 8- to 10-μm square-ended nuclear injection pipettes and piezo pulses or slit with 35- to 40-μm enucleation pipettes. Intact oocytes were used as controls. These oocytes were electroactivated to induce parthenogenesis and transferred to the oviducts of estrus-synchronized recipient gilts. After 5 to 7 days, the recipient uteri were flushed to collect embryos, and embryonic development (morula-blastocyst stage embryos / collected embryos) and survival (viable embryos / collected embryos) were determined. In total, 221 zona-punctured, 129 zona-slitted and 57 intact embryos were transplanted into four, two and two gilts, respectively. The efficiency of embryo recovery was similar in all groups (64.3% to 79.1%). However, the zona-penetrated and incised embryos exhibited unstable development and survival compared with the controls; development and survival of the control embryos were 94.7% and 87.7%, whereas those of the zona-punctured embryos were 69.0% and 47.9% (P < 0.01) and those of the zona-slit embryos were 64.7% and 50.0% (P<0.01). Cells with large foci that appeared to be macrophage giant cells were observed at the surface or inside the degenerated zona-damaged embryos. These results indicate that the recipientÅfs immune response to damage to the zona pellucida may impair embryonic development after transplantation to the oviduct. This may be one of the factors causing the reduced efficiency of live progeny production by SCNT.
- 日本繁殖生物学会の論文
- 2007-10-01
著者
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UENO Satoshi
Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji U
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NAGASHIMA Hiroshi
Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji U
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TOMII Ryo
Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji Un
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KUROME Mayuko
Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji Un
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Ueno Satoshi
Lab. Of Developmental Engineering Dep. Of Life Sciences School Of Agriculture Meiji Univ.
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HIRUMA Katsumi
Laboratory of Developmental Engineering Department of Life Science, School of Agriculture, Meiji Uni
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Nagashima Hiroshi
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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Tomii Ryo
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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Ueno Satoshi
Laboratory Of Developmental Engineering Department Of Life Science Meiji University
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Kurome Mayuko
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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Hiruma Katsumi
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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Saitoh Hitoshi
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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Nagashima Hiroshi
Lab. Of Developmental Engineering Dep. Of Life Sciences School Of Agriculture Meiji Univ.
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Nagashima Hiroshi
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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Kurome Mayuko
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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Nagashima Hiroshi
Laboratory For Evolutionary Morphology Riken Cdb
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